Anti-tsp23 Antibody (11137)

$457.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,AM Human, Mouse
SKU: 11137 Categories: , ,
Overview
Product Name Anti-tsp23 Antibody (11137)
Description Anti-tsp23 Mouse Monoclonal Antibody
Target tsp23
Species Reactivity Human, Mouse
Applications WB,IHC,AM
Host Mouse
Clonality Monoclonal
Clone ID tsp232A
Isotype IgG1
Immunogen Recombinant tsp23
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human and mouse tsp23. Other species have not been investigated. It does not cross-react with p23.
Target Name Putative protein PTGES3L
Target ID tsp23
Uniprot ID B9A003
Gene Name PTGES3L
Gene ID 80755
Accession Number NP_001136126.1
Research Areas Heat Shock& Stress Proteins
Background tsp23 (transcript similar p23) is a homolog of human p23, a highly conserved ubiquitous protein and a cochaperone for the Hsp90 chaperone system. A small protein (18-25kDa) with a simple structure, p23 is a highly acidic phosphoprotein with an aspartic acid- rich C-terminal domain. Although numerous studies have found p23 associated with other proteins (i.e, Fes tyrosine kinase, heme regulated kinase HRI, Hsf1 transcription factor, aryl hydrocarbon receptor, telomerase, and Hepadnavirus reverse transcriptase), the functional significance of p23 remains to be clearly defined. It appears to be involved in ATP-mediated Hsp90 binding of client proteins. A recent study suggests that p23 might be a promising target in leukemic apoptosis. tsp23 is 44% homologous with p23.
Application Images
Description Immunohistochemistry analysis using Mouse Anti-Tsp23 Monoclonal Antibody, Clone TSp232A (11137). Tissue: Heart Skeletal Muscle. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Tsp23 Monoclonal Antibody (11137) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Description Western Blot analysis of Human Heat Shocked cervical cancer cell line (HeLa) lysate showing detection of Tsp23 protein using Mouse Anti-Tsp23 Monoclonal Antibody, Clone TSp232A (11137). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Tsp23 Monoclonal Antibody (11137) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. A band of ~19kDa is detected.

These are recommended concentrations;

Enduser should determine optimal concentrations for their applications.

Positive control: Lysate of HeLa cells transiently transfected with Tsp23.
References & Data Sheet