Antibody Purification

QED will purify antibodies from serum, culture supernatant, or ascites fluid. Starting materials may be produced at our facility, or you can send your materials to us for purification.

Antibody Purification

Protein G or Protein A Affinity Chromatography

Serum or Ascites
Culture Supernatant
CL1000 or CL350 flasks
500ml
1 liter
5 liters

Antigen Affinity Chromatography

Affinity column, small
Affinity column, large  
Antigen Affinity Purification

We will advise on how much antigen is required for an affinity column based on the size/molecular weight of the peptide or protein antigen.

PEG Precipitation

PEG precipitation is the method used to purify mouse IgM and IgG3 monoclonal antibodies from ascites or culture supernatant since those antibody subclasses bind poorly to Protein G or Protein A.

1-100ml sample
101-1,000ml sample

Don’t see the service that you need? Have Questions?

Simply call us at 800-929-2114 — chances are we can help.