BriClone Hybridoma Cloning Medium
Product No.: BRI10000
Size: 100 ml
The traditional use of feeder cells to support the outgrowth of newly-created hybridomas has many disadvantages. Batch-to-batch variations may occur in the preparations of feeder cells, feeder cells are a potential source of contamination, and feeder cells may compete for vital nutrients with freshly-fused hybridomas. BriClone hybridoma cloning medium has been developed to overcome and eliminate these common problems.
BriClone is a conditioned medium collected from a proprietary human cell line that produces IL-6. It is supplied as a sterile frozen medium in 100 ml volumes. Each batch of BriClone is tested for its ability to support the outgrowth of hybridomas from freshly fused cells and for its ability to support the growth of hybridomas under conditions of limiting dilution. BriClone is guaranteed Mycoplasma-free.
For development of mouse and rat hybridoma cell lines. BriClone is an IL-6 enriched cloning medium for use in the post- fusion stages of hybridoma production and for cloning hybridoma cells. BriClone completely eliminates the need for feeder cells when used as a 5% v/v supplement in complete hybridoma culture medium. Each batch of BriClone is tested for its ability to support the growth of hybridomas from freshly fused cells and under conditions of limiting dilution.
INSTRUCTIONS FOR USE
Add BriClone Hybridoma Cloning Medium to hybridoma culture medim as a 5% (v/v) supplement. BriClone is suitable for use in the post-fusion stages of hybridoma production and when cloning hybridoma cell lines under conditions of limiting dilution. BriClone, when added to culture medium, eliminates the need for feeder cells in these types of cultures.
STORAGE AND STABILITY
Store at -20°C for up to six months.
This product is intended for in vitro research use only. BriClone is a product of National Cell and Tissue Culture Center, Dublin, Ireland.