Mycoplasma contamination of cell cultures comes from lab personnel or from contaminated culture media. Mycoplasma are too small to see with a conventional microscope and don’t change the pH of media or cause turbidity, but they can cause serious cellular changes and can ultimately destroy a cell line. The most common signs of Mycoplasma contamination are poor cell growth and/or death of a cell line after just a few days in culture. Working with a Mycoplasma-contaminated cell line poses great risk to other cell lines in a laboratory, so identifying a contaminated cell line is critical.
QED will prepare your cell lines from frozen stocks under quarantine conditions (3 passages in antibiotic-free medium) then test them by PCR for the presence of Mycoplasma sp. You will receive a complete report of the results and the procedures used.
|Preparation of cell lines for testing
Requires approx. 1 week.
|PCR Test||$200.00/cell line|
If you have a Mycoplasma-contaminated cell line, all is not lost! We partner with another laboratory that can decontaminate your cell line and deliver a cell bank of “clean” cells if you can provide a sample containing some live cells. Please contact us to inquire about this decontamination service.