Anti-Glutathione-S-Transferase (GST) Antibody (18803)

$503.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IP Glutathione-S-Transferase (Gst)
SKU: 18803 Categories: , ,
Overview
Product Name Anti-Glutathione-S-Transferase (GST) Antibody (18803)
Description Anti-GST Mouse Monoclonal Antibody
Target Glutathione-S-Transferase (GST)
Species Reactivity Glutathione-S-Transferase (Gst)
Applications WB,IP
Host Mouse
Clonality Monoclonal
Clone ID 3G10
Isotype IgG1
Immunogen GST protein of Schistosoma japonicum expressed in E. coli.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes GST.
Target Name Glutathione S-transferase class-mu 28 kDa isozyme
Target ID Glutathione-S-Transferase (GST)
Uniprot ID P26624
Alternative Names GST 28
Biological Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.; GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Research Areas Epitope Tags
Background A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids which, compared to tags such as the myc-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. Many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
Application Images
Description GST tag antibody [3G10] detects GST tag protein by Western blot analysis.
A. Non-induced cell lysate from E. coli expressing GST-tagged proteins
B. IPTG-induced cell lysate from E. coli expressing GST-tagged proteins
10 % SDS-PAGE
GST tag antibody [3G10] (18803) dilution: 1:10000
Description GST tag antibody [3G10] detects GST tag protein by Western blot analysis.
A. 2 ug His-recombinant protein
B. 2 ug GST-recombinant protein
C. 1 ug GST-recombinant protein
D. 0.5 ug GST-recombinant protein
10 % SDS-PAGE
GST tag antibody [3G10] (18803) dilution: 1:5000
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.2-2ug/mL.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet
References Mosser AG et al. 2002 J Infect Dis 185: 734.Mosser AG et al. 2005 Am J Respir Crit Care Med 171: 645.Jurgeit A et a. 2010 Virology J 7: 264.Chattoraj SS et al. 2011 Infect Immun 79: 4131. Not intended for diagnostic or therapeutic procedures.