GIT1 Monoclonal Antibody
Catalog No.: 56537 (clone S39B-8)
Size: 100ug in PBS (1mg/ml), pH 7.4, 50% glycerol, 0.09% sodium azide.
Purified by Protein G affinity chromatography.
G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains for small GTPases, kinases, the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT1 is localized to focal adhesions, cyto- plasmic complexes and membrane protrusions, and it regulates cell protrusion formation and cell migration.
Antigen: Fusion protein corresponding to aa 375-770 (C-terminus) of rat GIT1 (accession no.Q9Z272).
Host Species: Mouse
Antibody Class: IgG1
This antibody recognizes human, mouse and rat GIT1. It does not cross-react with GIT2.
Immunoblotting: use at 1ug/ml. Predicted molecular weight is ~90kDa.
Positive Control: rat brain lysate.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze- thaw cycles.
For in vitro investigational use only. Not intended for diagnostic or therapeutic applications.