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Anti-Chromogranin B Antibody (26102)

$530.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 200ul WB,IHC Human, Mouse, Rat
SKU: 26102 Categories: , ,
Overview
Product Name Anti-Chromogranin B Antibody (26102)
Description Anti-Chromogranin B Rabbit Polyclonal Antibody
Target Chromogranin B
Species Reactivity Human, Mouse, Rat
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide derived from the C-terminus (aa 641-655) of chromogranin B (accession no. NP_001810. This sequence is identical in human, mouse, and rat chromogranin B.
Properties
Form Liquid
Concentration Lot Specific
Formulation Whole antiserum
Buffer Formulation Whole Antiserum
Format Whole Antiserum
Purification Whole antiserum
Specificity Information
Specificity This antibody recognizes human, mouse, and rat chromogranin B.
Target Name Secretogranin-1
Target ID Chromogranin B
Uniprot ID P05060
Alternative Names Chromogranin-B, CgB, Secretogranin I, SgI [Cleaved into: PE-11; GAWK peptide; CCB peptide]
Gene Name CHGB
Sequence Location Secreted. Note=Neuroendocrine and endocrine secretory granules.
Biological Function Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
Research Areas Hormones/Proteins
Background Chromogranin B (CgB, secretogranin I) is a secretory granule matrix protein expressed in a wide variety of endocrine cells and neurons where it may have both extracellular and intracellular functions. Extracellular roles for CgB are dependent on its proteolytic processing within secretory granules to form smaller peptides that may have a role in the neuroendocrine/sympathoadrenal stress response to systemic infection, and CgB fragments may also have an autocrine inhibitory role in the release of co-stored hormones, such as insulin.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C to -70°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use diluted 1:2,500- 1:10,000. A band of 110kDa is detected. Although the predicted mw of CgB is ~76kDa, the highly acidic nature of CgB causes it to run at a higher mw in SDS- PAGE.

Immunohistochemistry: use diluted 1:500- 1:5,000.
References & Data Sheet
References Thompson ME et al. 1992. Prolactin granulogenesis is associated with increased secretogranin expression and aggregation in the Golgi apparatus of GH4C1 cells. Endocrinology 131: 318-326.
PMID 1612012