Mikrogen Diagnostik recomWell Chlamydia pneumoniae IgG ELISA Kit (6104)

$462.00

Host Quantity Applications Species Reactivity Data Sheet  
96 Tests ELISA Chlamydia pneumoniae
SKU: 6104 Categories: ,
Overview
Product Name Mikrogen Diagnostik recomWell Chlamydia pneumoniae IgG ELISA Kit (6104)
Description Enzyme immunoassay based on Chlamydia pneumoniae specific antigen (COMC) for the detection of IgG, IgM or IgA antibodies against Chlamydia pneumoniae in human serum and plasma

Product is intended for research use.
Target Human Chlamydia pneumoniae IgG
Species Reactivity Chlamydia pneumoniae
Assay Type Indirect ELISA
Applications ELISA
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recomWell Chlamydia pneumoniae IgA ELISA Kit (6106)
recomLine Chlamydia IgG Lateral Strip Test Kit (6172)
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Properties
Background Chlamydia pneumoniae is a widespread, obligately intracellular bacterium which affects the respiratory tract. In Germany the prevalence in women and men in the sixth decade of life is estimated >50% and >70% respectively. Most infections are asymptomatic, a few infections cause mild symptoms like sinusitis or bronchitis. Nevertheless, we also see severe courses of infections associated with asthma, pneu-moniae, rarely coming along with arthritis or atherosclerosis. Chlamydiae reproduce in a biphasic development cycle showing extracellular, infectious elementary bodies and intracellular, metabolically active but not infectious reticular bodies. This kind of reproduction facilitates persistence formation and chronical infection.In diagnostics direct detection of chlamydia antigen (cultivation and PCR techniques) and indirect proof by antibody detection are used. Cultivation of Chlamydiae however is quite time-consuming and PCR is not well standardized. Reliable differentiation between acute and persistent infection remains difficult. In Chlamydia pneumoniae serology the Micro Immune Fluorescence (MIF) assay is considered as the gold standard. This technique is labor-intensive, requires experience and is therefore reserved for special laboratories. To avoid unnecessary antibiotic therapy there is a need to provide accurate analytics for routine diagnostics to be guided by the gold standard MIF and reflecting the clinical situation.To detect antibodies against the pathogen the recomWell Chlamydia pneumoniae is using antigens from the outer membrane complex of Chlamydia pneumoniae (COMC). With the objective to discriminate mild, clini-cally inconspicuous infections from clinically relevant infections the test was built up on a MIF cut-off value of ≥1:32. The recomWell Chlamydia pneumoniae shows an excellent specificity compared to other commercially available ELISA test systems. In healthy blood donors the recomWell Chlamydia pneumoniae detects an IgG prevalence of 39.5% and an IgA prevalence of 4.5% respectively.
Sample Type Serum, plasma, whole blood
Assay Resolution See Figure
Components
10X Wash Buffer100 mL
Dilution Buffer125 mL
TMB Substrate12 mL
Stop Slution12 mL
Instuctions for Use1 Each
Evaluation Form1 Each
Microplate Sealing Tape2 Each
Microtiter Plate12 x 8 Wells
Positive Control450 uL
Cut-Off Control450 uL
Negative Control450 uL
Anti-Human IgG Conjugate500 uL
Specificity Information
Target ID Human Chlamydia pneumoniae IgG
Research Areas Infectious Disease
Application Images
Description Test principle and procedure.
Description Test evaluation data.
Additional Information
Handling
Storage Store at 2-8°C.
References & Data Sheet