ELISA format based on Chlamydia pneumoniae specific antigen (COMC) for detection of IgG, IgM, or IgA antibodies to Chlamydia pneumoniae.
Chlamydia pneumoniae is an obligate intracellular bacterium that targets the respiratory tract. Although most infections are asymptomatic or mildly symptomatic, more severe infections do occur, often in elderly people.
Chlamydiae reproduce in a biphasic development cycle: extracellular infectious elementary bodies and intracellular metabolically active non-infectious reticular bodies. This type of reproduction facilitates persistent, chronic infections.
recomWell Chlamydia pneumoniae are highly-specific assays that incorporate recombinant antigens of the outer membrane complex of Chlamydia pneumoniae (COMC) elementary bodies and reticular bodies.
• High sensitivity and specificity.
• Excellent discrimination between negative and positive results.
• Separate detection of IgG, IgM, and IgA antibodies is possible.
• Simple, user-friendly assay procedure.
• Assay procedure and reagents identical in all MIKROGEN recomWell products – reagents exchangeable.
Test principle and procedure
Store products at 2-8oC.
Product no. 6104
recomWell Chlamydia pneumoniae IgG (Reagents for 96 determinations)
Product no. 6105
recomWell Chlamydia pneumoniae IgM
Product no. 6106
recomWell ChIamydia pneumoniae IgA (Reagents for 96 determinations)
Indirect ELISA format. Recombinant antigens are bound to the solid phase.
1st Incubation Add samples (10μl of serum or plasma) diluted 1:100, incubate for 1 h at 37 °C.
Wash 4 times.
2nd Incubation Add peroxidase conjugated anti-human IgG, IgM, or IgA antibodies (conjugate), incubate for 30 min at 37 °C.
Wash 4 times.
Color reaction Add ready-to-use TMB solution and incubate for 30 min at room temperature. Stop the substrate reaction with H3PO4 and measure the extinction at 450 nm.
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Available in the U.S. For Research Use Only.
Not intended for diagnostic procedures.