Beta-Actin Polyclonal Antibody
Catalog No.: 56574
Format: 100ug (1mg/ml) peptide affinity-purified antibody in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Actins are essential components of the cytoskeleton with critical roles in a wide range of cellular processes including cell migration, cell division, and the regulation of gene expression. Beta-actin, product of the Actb gene, is one of six different actin isoforms and is one of the two nonmuscle cytoplasmic actins (gamma-actin is the other); both are ubiquitously expressed and highly conserved from birds to mammals. Mutations in the Actb gene have been associated with diffuse large B-cell lymphoma. Beta-actin (molecular weight ~42kDa) is often used as a loading control in Western blots.
Antigen: Synthetic peptide corresponding to mid-protein amino acids of human beta-actin.
Accession no.: NP_001092.1, P60709
Gene ID: 60
Host Species: Rabbit
Specificity: This antibody recognizes human beta-actin.
Immunoblotting: use at 1ug/ml.
Immunofluorescence: use at 10ug/ml.
These are recommended concentrations; endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C.
For in vitro investigational use only. Not intended for diagnostic or therapeutic applications.