Anti-UVRAG Antibody (23013)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ICC/IF Human, Rat
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SKU: 23013 Categories: , ,
Overview
Product Name Anti-UVRAG Antibody (23013)
Description Anti-UVRAG Rabbit Polyclonal Antibody
Target UVRAG
Species Reactivity Human, Rat
Applications WB,ICC/IF
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to mid-protein amino acids of human UVRAG.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Concentration: 1mg/ml
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody recognizes human and rat UVRAG.
Target Name UV radiation resistance-associated gene protein
Target ID UVRAG
Uniprot ID Q9P2Y5
Alternative Names p63
Gene Name UVRAG
Sequence Location Late endosome, Lysosome, Cytoplasmic vesicle, autophagosome, Early endosome, Endoplasmic reticulum, Midbody, Chromosome, centromere
Biological Function Versatile protein that is involved in regulation of different cellular pathways implicated in membrane trafficking. Involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; the function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns(3)P) (PubMed:16799551, PubMed:18552835, PubMed:20643123, PubMed:24056303, PubMed:28306502). During autophagy acts as regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. Activates lipid kinase activity of PIK3C3 (PubMed:16799551, PubMed:20643123, PubMed:24056303, PubMed:28306502). Involved in the regulation of degradative endocytic trafficking and cytokinesis, and in regulation of ATG9A transport from the Golgi to the autophagosome; the functions seems to implicate its association with PI3KC3-C2 (PubMed:16799551, PubMed:20643123, PubMed:24056303). Involved in maturation of autophagosomes and degradative endocytic trafficking independently of BECN1 but depending on its association with a class C Vps complex (possibly the HOPS complex); the association is also proposed to promote autophagosome recruitment and activation of Rab7 and endosome-endosome fusion events (PubMed:18552835, PubMed:28306502). Enhances class C Vps complex (possibly HOPS complex) association with a SNARE complex and promotes fusogenic SNARE complex formation during late endocytic membrane fusion (PubMed:24550300). In case of negative-strand RNA virus infection is required for efficient virus entry, promotes endocytic transport of virions and is implicated in a VAMP8-specific fusogenic SNARE complex assembly (PubMed:24550300). {PubMed:18552835, PubMed:20643123, PubMed:24056303, PubMed:28306502, PubMed:22542840}.
Research Areas Autophagy
Background Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. UV radiation resistance-associated gene product (UVRAG) is a protein localized in the endoplasmic reticulum (ER) and endosomes and is known to regulate autophagosome maturation as well as early stages of autophagy. UVRAG regulates autophagosome maturation by binding to the homotypic fusion and vacuole protein sorting (HOPS) complex, which consists of the class C Vps complex (Vps11-Vps16-Vps18-Vps33) and two additional proteins, Vps39 and Vps41. UVRAG binding to the HOPS complex stimulates lysosomal fusion with autophagosome and endosome.
Application Images
Description Immunoblotting: use at 12ug/ml. A band of ~7590kDa is detected. Detection of UVRAG in 20ug (2) HeLa and (3) 293T cell lysate.
Description Detection of UVRAG in 20ug rat liver lysate.
Description Immunofluorescence: use at 10ug/ml. Detection of UVRAG in formaldehyde-fixed neuroblastoma SK-N-BE cells.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1- 2ug/mL. A band of ~75- 90kDa is detected.

Immunofluorescence: use at 10ug/mL.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet