Anti-SODD (NT) Antibody (2143)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human, Mouse, Rat
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Overview
Product Name Anti-SODD (NT) Antibody (2143)
Description Anti-SODD (NT) Rabbit Polyclonal Antibody
Target SODD (NT)
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic peptide corresponding to aa 2-16 of human SODD (accession no. AF111116).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat SODD (60kDa).
Target Name BAG family molecular chaperone regulator 4
Target ID SODD (NT)
Uniprot ID O95429
Alternative Names BAG-4, Bcl-2-associated athanogene 4, Silencer of death domains
Gene Name BAG4
Gene ID 9530
Accession Number NP_004865
Sequence Location Cytoplasm.
Biological Function Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release (By similarity). Prevents constitutive TNFRSF1A signaling. Negative regulator of PRKN translocation to damaged mitochondria. {PubMed:24270810}.
Research Areas Apoptosis
Background A novel TNF-R1-interacting protein has been identified and designated SODD (silencer of death domains). SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF- R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD to engage in the active TNF-R1 complex, thus activating TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.
Application Images
Description Western blot analysis of SODD in HeLa (1,3) and THP-1 (2,4) whole cell lysates in the absence (1,2) or presence (3,4) of blocking peptide (Catalog no. 2143P) with SODD antibody at 1:500 dilution.
Description Immunocytochemistry of SODD in HeLa cells with SODD antibody at 5 ug/mL.
Description Immunofluorescence of SODD in Hela cells with SODD antibody at 20 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting : use at 1ug/mL.

Positive control: HeLa or THP-1 cell lysate.

Immunocytochemistry: use at 5ug/mL

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet