Anti-Smac (CT) Antibody
Catalog No.: 1409
Size: 100ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
Inhibitors of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases has been identified and designated Smac/DIABLO. Smac is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf- 1/caspase-9 pathway. The N-terminal amino acids of Smac are required for binding to IAPs and for activation of caspases. Smac is expressed in a variety of human and mouse tissues.
Antigen: Synthetic peptide corresponding to aa 225-239 of human Smac (accession no. AAF87716).
Host Species: Rabbit
Preservatives: 0.02% sodium azide.
This antibody recognizes human, mouse, and rat Smac (25kDa).
Immunoblotting: use at 1ug/ml.
Positive control: Human heart tissue lysate.
Immunohistochemistry: use at 5ug/ml
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.