Anti-RICK (CT) Antibody (2183)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human, Mouse, Rat
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Overview
Product Name Anti-RICK (CT) Antibody (2183)
Description Anti-RICK (CT) Rabbit Polyclonal Antibody
Target RICK (CT)
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic peptide corresponding to aa 508-522 of human RICK (accession no. AF027706).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat RICK (60kDa).
Target Name Receptor-interacting serine/threonine-protein kinase 2
Target ID RICK (CT)
Uniprot ID O43353
Alternative Names EC 2.7.11.1, CARD-containing interleukin-1 β-converting enzyme-associated kinase, CARD-containing IL-1 β ICE-kinase, RIP-like-interacting CLARP kinase, Receptor-interacting protein 2, RIP-2, Tyrosine-protein kinase RIPK2, EC 2.7.10.2
Gene Name RIPK2
Gene ID 8767
Accession Number NP_003812
Sequence Location Cytoplasm
Biological Function Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation. Plays a role in the inactivation of RHOA in response to NGFR signaling (PubMed:26646181). {PubMed:14638696, PubMed:17054981, PubMed:18079694, PubMed:21123652, PubMed:21887730, PubMed:26646181}.
Research Areas Apoptosis
Background A CARD-containing serine/threonine kinase has been identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively. RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induces apoptosis and activates NF- B and JNK. RICK interacts with members of the TRAF family, CLARP, and caspase-1. RICK regulates TNF and Fas induced-apoptosis and is involved in the generation of the proinflammatory cytokine IL-1 . The mRNA of RICK is expressed in many human tissues.
Application Images
Description Western blot analysis of RICK in (A) 3T3 and (B) K562 cell lysate with RICK antibody at 0.5 ug/mL
Description Immunocytochemistry of RICK in K562 cells with RICK antibody at 5 ug/mL.
Description Immunofluorescence of RICK in K562 cells with RICK antibody at 20 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting : use at 1-2ug/mL.

Positive control: A431 cell lysate.

Immunocytochemistry: use at 5ug/mL.
References & Data Sheet