Mikrogen Diagnostik recomLine HantaPlus IgM Lateral Strip Test Kit (7673)
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 20 Tests | Lateral Strip Assay | Hanta Virus |  |
Overview
Product Name Mikrogen Diagnostik recomLine HantaPlus IgM Lateral Strip Test Kit (7673)
Description Strip-Immunoassay with antigens produced by recombinant techniques for the detection of IgG or IgM antibodies against Hantavirus (serotypes Puumala (PuN), Sin Nombre (SinN), Hantaan (HaN), Dobrava (DobN) and Seoul (SeoN)) as well as Sandfly Fever Virus (SFFV) serotypes Toscana and Sicilian. Includes markers:
PuN
SinN
HaN
DobN
SeonN
SFFV
PuN
SinN
HaN
DobN
SeonN
SFFV
Target Human Hanta Virus IgM
Species Reactivity Hanta Virus
Assay Type Indirect Sandwich ELISA
Applications Lateral Strip Assay
Properties
Background Most of the Bunyaviruses can be found in the tropics and subtropics. An exception are the genera of Hantavirus und Phlebovirus which are also represented in Europe as well.
Hantavirus:
Different rodent species are vectors for the different hantavirus serotypes Puumala, Sin Nombre, Dobrava, Hantaan and Seoul. Many other serotypes are described in literature to be found in isolated areas. Based on a significant homology within the N-terminal part of the virus nucleocapsid antigen, hantaviruses can be devided in two serological groups, Puumala and Sin Nombre, and the group of Hantaan, Dobrava and Seoul. Hantaviruses are transmitted via stirred and inhaled dust, contaminated with rodents droppings. Hantavirus outbreaks regularly monitored every two to three years are very likely interconnected with an excessive food supply for the vector animals (rodents) and their proliferation. Typical peaks in case numbers can be related to holiday seasons and spring-cleaning activities, like cleaning basement, outbuilding or summer houses with unattended rodent infestation. There are no cases described for human to human transmission. The onset of a hantavirus infection is characterized by flu-like symptoms. The Puumalavirus prevelant in Europe can cause renal failures requiring hemodialysis and intensive care treatment. This Nephropathia epidemica (NE), describes a moderate form of the hemorrhagic fever with renal syndrom (HFRS), caused by infections with the virus types Dobrava, Hantaan and Seoul. The lethality of a Puumala infection is reported to be less than 1%. An infection with Sin Nombre or Andes virus may result in a severe Hantavirus Pulmonary Syndrom (HPS) with lethality rates reported over 30%.
Sandfly Fever Virus (SFFV):
A few Bunyaviruses in Europe are transmitted by the Sandfly (Phlebotomus pappatasi), which is present in the mediterranean area. Sandfly Fever, synonym Pappatasi Fever, is characterized by fever symptoms and headache. More severe courses with fever, strong, frontal headache and an aseptic meningitis with neurological symptoms are described for infections with the serotypes Toscana and Sicilian.
Hantavirus:
Different rodent species are vectors for the different hantavirus serotypes Puumala, Sin Nombre, Dobrava, Hantaan and Seoul. Many other serotypes are described in literature to be found in isolated areas. Based on a significant homology within the N-terminal part of the virus nucleocapsid antigen, hantaviruses can be devided in two serological groups, Puumala and Sin Nombre, and the group of Hantaan, Dobrava and Seoul. Hantaviruses are transmitted via stirred and inhaled dust, contaminated with rodents droppings. Hantavirus outbreaks regularly monitored every two to three years are very likely interconnected with an excessive food supply for the vector animals (rodents) and their proliferation. Typical peaks in case numbers can be related to holiday seasons and spring-cleaning activities, like cleaning basement, outbuilding or summer houses with unattended rodent infestation. There are no cases described for human to human transmission. The onset of a hantavirus infection is characterized by flu-like symptoms. The Puumalavirus prevelant in Europe can cause renal failures requiring hemodialysis and intensive care treatment. This Nephropathia epidemica (NE), describes a moderate form of the hemorrhagic fever with renal syndrom (HFRS), caused by infections with the virus types Dobrava, Hantaan and Seoul. The lethality of a Puumala infection is reported to be less than 1%. An infection with Sin Nombre or Andes virus may result in a severe Hantavirus Pulmonary Syndrom (HPS) with lethality rates reported over 30%.
Sandfly Fever Virus (SFFV):
A few Bunyaviruses in Europe are transmitted by the Sandfly (Phlebotomus pappatasi), which is present in the mediterranean area. Sandfly Fever, synonym Pappatasi Fever, is characterized by fever symptoms and headache. More severe courses with fever, strong, frontal headache and an aseptic meningitis with neurological symptoms are described for infections with the serotypes Toscana and Sicilian.
Sample Type Serum, plasma, whole blood
Assay Resolution See Figure
Components
| 10X Wash Buffer | 100 mL |
| TMB Substrate | 40 mL |
| Milk Powder | 5 g |
| Instuctions for Use | 1 Each |
| Evaluation Form | 1 Each |
| Test Strips | 2 Vials x 10 Each |
| Anti-Human IgM Conjugate | 500 uL |
| Positive Control | 140 uL |
| Negative Control | 140 uL |
Specificity Information
Target Name Hanta Virus markers: Puumala virus nucleocapsid, Sin Nombre virus nucleocapsid, Hantaan virus nucleocapsid, Dobrava virus nucleocapsid, Seoul virus nucelocapsid, Sandfly fever nucleocapsid.
Target ID Human Hanta Virus IgM
Alternative Names PuN, SinN, HaN, DobN, SeonN, SFFV
Research Areas Infectious Disease
Application Images
Description Test Strip Layout
Description Test Principle
Description Evaluation
Description Evaluation
Additional Information
Additional Information Additional Mirkogen Kit Information
Handling
Storage Store at 2 - 8°C until expiration on packaging.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet