Recombinant Human Mn Superoxide Dismutase (SOD2)

$173.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
	5ug	WB	Human

SKU: 13003P-5 Categories: Products, Recombinant Proteins

Overview

Product Name Mn Superoxide Dismutase (13003P-5)

Description Recombinant Human Mn SOD2 produced in E. coli is a single, non-glycosylated polypeptide chain containing 219 amino acidsaa 25-222) with a molecular weight of 24.4kDa. It has a His- tag at the N-terminus.

Target Mn Superoxide Dismutase

Product Background SOD2 is part of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. SOD2 binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in SOD2 gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. SOD2 destroys radicals which are usually produced within the cells and which are toxic to biological systems.

Species Reactivity Human

Applications WB

Source Escherichia coli

Properties

Form Liquid

Concentration Lot Specific

Formulation Sterile-filtered solution in 20mM Tris-HCl, pH 8.0, 20% glycerol.

Buffer Formulation 20 mM Tris

Buffer pH pH 8.0

Buffer Cryopreservative 20% glycerol

Purity Greater than 95% as determined by SDS- PAGE

Target Name Superoxide dismutase [Mn], mitochondrial

Target ID Mn Superoxide Dismutase

Alternative Names rHuMnSOD (SOD2)

Sequence Location Mitochondrion matrix.

Sequence MGSSHHHHHH SSGLVPRGSH MKHSLPDLPY DYGALEPHIN AQIMQLHHSK HHAAYVNNLN VTEEKYQEAL AKGDVTAQIA LQPALKFNGG GHINHSIFWT NLSPNGGGEP KGELLEAIKR DFGSFDKFKE KLTAASVGVQ GSGWGWLGFN KERGHLQIAA CPNQDPLQGT TGLIPLLGID VWEHAYYLQY KNVRPDYLKA IWNVINWENV TERYMACKK.

Biological Activity Mn Superoxide Dismutase

Biological Function Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. {PubMed:10334867}.

Background SOD2 is part of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. SOD2 binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in SOD2 gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. SOD2 destroys radicals which are usually produced within the cells and which are toxic to biological systems.

Handling

Storage Store at 2 - 8ºC. Do not Freeze.

Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.

Data Sheet