Recombinant Human HspH1 (Hsp105)

Recombinant Human HspH1 (Hsp105)

ORDERING INFORMATION

Formulation: Sterile-filtered colorless solution in 20mM Tris-HCl, pH 8.0, and 50mM NaCl. Purified by proprietary chromatographic techniques.

BACKGROUND

HspH1 is a member of the mammalian Hsp105/110 family, a subgroup of the Hsp70 family. Two isoforms of HspH1, alpha and beta, have been identified. HspH1 associates with Hsp70/Hsc70 as complexes and negatively regulates the chaperone activity of Hsp70/Hsc70 in vivo and in vitro. HspH1 is highly expressed in many human tumors.

DESCRIPTION

Recombinant human HspH1 produced in E. coli is a single, non-glycosylated polypeptide containing 894 amino acids with a molecular weight of 100kDa. It is expressed with a His-tag at the N- terminus.

SPECIFICATION SUMMARY

Source: Escherichia coli
Purity: Greater than 90% as determined by SDS-PAGE and RP- HPLC.
Accession number: Q92598
Amino acid sequence:
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSVV GLDVGSQSCY IAVARAGGIE TIANEFSDRC TPSVISFGSK NRTIGVAAKN QQITHANNTV SNFKRFHGRA FNDPFIQKEK ENLSYDLVPL KNGGVGIKVM YMGEEHLFSV EQITAMLLTK LKETAENSLK KPVTDCVISV PSFFTDAERR SVLDAAQIVG LNCLRLMNDM TAVALNYGIY KQDLPSLDEK PRIVVFVDMG HSAFQVSACA FNKGKLKVLG TAFDPFLGGK NFDEKLVEHF CAEFKTKYKL DAKSKIRALL RLYQECEKLK KLMSSNSTDL PLNIECFMND KDVSGKMNRS QFEELCAELL QKIEVPLYSL LEQTHLKVED VSAVEIVGGA TRIPAVKERI AKFFGKDIST TLNADEAVAR GCALQCAILS PAFKVREFSV TDAVPFPISL IWNHDSEDTE GVHEVFSRNH AAPFSKVLTF LRRGPFELEA FYSDPQGVPY PEAKIGRFVV QNVSAQKDGE KSRVKVKVRV NTHGIFTIST ASMVEKVPTE ENEMSSEADM ECLNQRPPEN PDTDKNVQQD NSEAGTQPQV QTDAQQTSQS PPSPELTSEE NKIPDADKAN EKKVDQPPEA KKPKIKVVNV ELPIEANLVW QLGKDLLNMY IETEGKMIMQ DKLEKERNDA KNAVEEYVYE FRDKLCGPYE KFICEQDHQN FLRLLTETED WLYEEGEDQA KQAYVDKLEE LMKIGTPVKV RFQEAEERPK MFEELGQRLQ HYAKIAADFR NKDEKYNHID ESEMKKVEKS VNEVMEWMNN VMNAQAKKSL DQDPVVRAQE IKTKIKELNN TCEPVVTQPK PKIESPKLER TPNGPNIDKK EEDLEDKNNF GAEPPHQNGE CYPNEKNSVN MDLD.

STORAGE AND STABILITY

Store at 4°C if entire vial will be used within 2-4 weeks. Store at or below – 20oC for longer periods of time. Addition of a carrier protein (such as 0.1% HSA or BSA) is recommended for long-term storage.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.