HspH1 (11124P-5)
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 5ug | WB | Human |  |
Overview
Product Name HspH1 (11124P-5)
Description Recombinant human HspH1 produced in E. coli is a single, non-glycosylated polypeptide containing 894 amino acids with a molecular weight of 100kDa. It is expressed with a His-tag at the N- terminus.
Target HspH1
Species Reactivity Human
Applications WB
Source Escherichia coli
Properties
Form Liquid
Concentration Lot Specific
Formulation Sterile-filtered colorless solution in 20mM Tris-HCl, pH 8.0, and 50mM NaCl.
Buffer Formulation 20 mM Tris
Buffer pH pH 8.0
Purity Greater than 90% as determined by SDS-PAGE and RP- HPLC
Background HspH1 is a member of the mammalian Hsp105/110 family, a subgroup of the Hsp70 family. Two isoforms of HspH1, alpha and beta, have been identified. HspH1 associates with Hsp70/Hsc70 as complexes and negatively regulates the chaperone activity of Hsp70/Hsc70 in vivo and in vitro. HspH1 is highly expressed in many human tumors.
Specificity Information
Target Name Heat shock protein 105 kDa
Target ID HspH1
Alternative Names Hsp105
Sequence Location Cytoplasm
Sequence MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSVV GLDVGSQSCY IAVARAGGIE TIANEFSDRC TPSVISFGSK NRTIGVAAKN QQITHANNTV SNFKRFHGRA FNDPFIQKEK ENLSYDLVPL KNGGVGIKVM YMGEEHLFSV EQITAMLLTK LKETAENSLK KPVTDCVISV PSFFTDAERR SVLDAAQIVG LNCLRLMNDM TAVALNYGIY KQDLPSLDEK PRIVVFVDMG HSAFQVSACA FNKGKLKVLG TAFDPFLGGK NFDEKLVEHF CAEFKTKYKL DAKSKIRALL RLYQECEKLK KLMSSNSTDL PLNIECFMND KDVSGKMNRS QFEELCAELL QKIEVPLYSL LEQTHLKVED VSAVEIVGGA TRIPAVKERI AKFFGKDIST TLNADEAVAR GCALQCAILS PAFKVREFSV TDAVPFPISL IWNHDSEDTE GVHEVFSRNH AAPFSKVLTF LRRGPFELEA FYSDPQGVPY PEAKIGRFVV QNVSAQKDGE KSRVKVKVRV NTHGIFTIST ASMVEKVPTE ENEMSSEADM ECLNQRPPEN PDTDKNVQQD NSEAGTQPQV QTDAQQTSQS PPSPELTSEE NKIPDADKAN EKKVDQPPEA KKPKIKVVNV ELPIEANLVW QLGKDLLNMY IETEGKMIMQ DKLEKERNDA KNAVEEYVYE FRDKLCGPYE KFICEQDHQN FLRLLTETED WLYEEGEDQA KQAYVDKLEE LMKIGTPVKV RFQEAEERPK MFEELGQRLQ HYAKIAADFR NKDEKYNHID ESEMKKVEKS VNEVMEWMNN VMNAQAKKSL DQDPVVRAQE IKTKIKELNN TCEPVVTQPK PKIESPKLER TPNGPNIDKK EEDLEDKNNF GAEPPHQNGE CYPNEKNSVN MDLD.
Biological Function Acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering client/substrate protein release (PubMed:24318877). Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities (By similarity). {UniProtKB:Q60446, UniProtKB:Q61699, PubMed:24318877}.
Background HspH1 is a member of the mammalian Hsp105/110 family, a subgroup of the Hsp70 family. Two isoforms of HspH1, alpha and beta, have been identified. HspH1 associates with Hsp70/Hsc70 as complexes and negatively regulates the chaperone activity of Hsp70/Hsc70 in vivo and in vitro. HspH1 is highly expressed in many human tumors.
Handling
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store at or below - 20°C for longer periods of time. Addition of a carrier protein (such as 0.1% HSA or BSA) is recommended for long-term storage.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet