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Annexin A7 (13822P-5)

Host Quantity Applications Species Reactivity Data Sheet  
5ug WB Human
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SKU: 13822P-5 Categories: ,
Overview
Product Name Annexin A7 (13822P-5)
Description Recombinant Human Annexin A7 produced in E. coli is a single, non- glycosylated polypeptide containing amino acids 1-466 with a molecular weight of 52.9kDa. It includes a 24 amino acid His-tag at the N-terminus.
Target Annexin A7
Species Reactivity Human
Applications WB
Source Escherichia coli
Properties
Form Liquid
Concentration Lot Specific
Formulation Sterile-filtered clear solution (0.25mg/ml) in 20mM Tris-HCl, pH 8.0, 150mM NaCl,1mM DTT, 40% glycerol. 
Buffer Formulation 20 mM Tris
Buffer pH pH 8.0
Buffer Cryopreservative 40% glycerol
Purity Greater than 85% as determined by SDS-PAGE
Background Annexin 7 is a member of the annexin family of calcium-dependent phospholipids binding proteins. It includes a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter is composed of alternating hydrophobic and hydrophilic segments. Analysis of this protein suggests that Annexin 7 is a membrane binding protein with diverse properties including voltage-sensitive calcium channel activity, ion selectivity, and membrane fusion.
Specificity Information
Target Name Annexin A7
Target ID Annexin A7
Alternative Names Annexin A7
Sequence MGSSHHHHHH SSGLVPRGSH
Biological Function Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
Background Annexin 7 is a member of the annexin family of calcium-dependent phospholipids binding proteins. It includes a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter is composed of alternating hydrophobic and hydrophilic segments. Analysis of this protein suggests that Annexin 7 is a membrane binding protein with diverse properties including voltage-sensitive calcium channel activity, ion selectivity, and membrane fusion.
Application Images
Additional Information
Handling
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store at -20°C for longer periods of time. For long-term storage, addition of 0.1% human serum albumin or bovine serum albumin is recommended. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
References & Data Sheet