Recombinant Human Alpha-1-Antitrypsin (A1AT)

Recombinant Human Alpha-1-Antitrypsin (A1AT)

ORDERING INFORMATION

Formulation: Sterile-filtered colorless solution contains 20mM Tris-HCl, pH 7.5, 1mM DTT, 10% glycerol, and 2mM EDTA. Purified by proprietary chromatographic techniques.

BACKGROUND

Alpha-1-antitrypsin (A1AT) is a serine protease inhibitor that inhibits the catalytic domain of elastase, plasmin, collagenase, thrombin, leucocytic proteases, trypsin, chymotrypsin, and plasminogen activator. Defects in the A1AT gene can lead to emphysema or liver disease. Increased levels of serum A1AT are found in cases of lung and prostate cancers and as an acute phase response to tissue necrosis and inflammation.

DESCRIPTION

Recombinant Human A1AT produced in E. coli is a single, non-glycosylated polypeptide chain containing 393 amino acids (aa 25-418) with a molecular weight of 44.4kDa.

SPECIFICATION SUMMARY

Source: Escherichia coli Purity: Greater than 90% as determined by SDS-PAGE. Accession number: P01009 Amino Acid Sequence:
MEDPQGDAAQ KTDTSHHDQD HPTFNKITPN LAEFAFSLYR QLAHQSNSTN IFFSPVSIAT AFAMLSLGTK ADTHDEILEG LNFNLTEIPE AQIHEGFQEL LRTLNQPDSQ LQLTTGNGLF LSEGLKLVDK FLEDVKKLYH SEAFTVNFGD TEEAKKQIND YVEKGTQGKI VDLVKELDRD TVFALVNYIF FKGKWERPFE VKDTEEEDFH VDQVTTVKVP MMKRLGMFNI QHCKKLSSWV LLMKYLGNAT AIFFLPDEGK LQHLENELTH DIITKFLENE DRRSASLHLP KLSITGTYDL KSVLGQLGIT KVFSNGADLS GVTEEAPLKL SKAVHKAVLT IDEKGTEAAG AMFLEAIPMS IPPEVKFNKP FVFLMIDQNT KSPLFMGKVV NPTQK.

STORAGE AND STABILITY

Although stable at 4°C for 4 weeks, product is best stored at or below -20°C. For long-term storage, addition of a carrier protein (such as 0.1% HSA or BSA) is recommended.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.