Recombinant Human Alpha-1-Antitrypsin (A1AT)

$294.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
	25ug	WB	Human

SKU: 13701P-25 Categories: Products, Recombinant Proteins

Overview

Product Name Alpha-1-Antitrypsin (13701P-25)

Description Recombinant Human A1AT produced in E. coli is a single, non-glycosylated polypeptide chain containing 393 amino acidsaa 25-418) with a molecular weight of 44.4kDa.

Target Alpha-1-Antitrypsin

Product Background Alpha-1-antitrypsin (A1AT) is a serine protease inhibitor that inhibits the catalytic domain of elastase, plasmin, collagenase, thrombin, leucocytic proteases, trypsin, chymotrypsin, and plasminogen activator. Defects in the A1AT gene can lead to emphysema or liver disease. Increased levels of serum A1AT are found in cases of lung and prostate cancers and as an acute phase response to tissue necrosis and inflammation.

Species Reactivity Human

Applications WB

Source Escherichia coli

Properties

Form Liquid

Concentration Lot Specific

Formulation Sterile-filtered colorless solution contains 20mM Tris-HCl, pH 7.5, 1mM DTT, 10% glycerol, and 2mM EDTA.

Buffer Formulation 20 mM Tris

Buffer pH pH 7.5

Buffer Cryopreservative 10% glycerol

Purity Greater than 90% as determined by SDS-PAGE

Target Name Alpha-1-antitrypsin

Target ID Alpha-1-Antitrypsin

Alternative Names A1AT

Sequence MEDPQGDAAQ KTDTSHHDQD HPTFNKITPN LAEFAFSLYR QLAHQSNSTN IFFSPVSIAT AFAMLSLGTK ADTHDEILEG LNFNLTEIPE AQIHEGFQEL LRTLNQPDSQ LQLTTGNGLF LSEGLKLVDK FLEDVKKLYH SEAFTVNFGD TEEAKKQIND YVEKGTQGKI VDLVKELDRD TVFALVNYIF FKGKWERPFE VKDTEEEDFH VDQVTTVKVP MMKRLGMFNI QHCKKLSSWV LLMKYLGNAT AIFFLPDEGK LQHLENELTH DIITKFLENE DRRSASLHLP KLSITGTYDL KSVLGQLGIT KVFSNGADLS GVTEEAPLKL SKAVHKAVLT IDEKGTEAAG AMFLEAIPMS IPPEVKFNKP FVFLMIDQNT KSPLFMGKVV NPTQK.

Background Alpha-1-antitrypsin (A1AT) is a serine protease inhibitor that inhibits the catalytic domain of elastase, plasmin, collagenase, thrombin, leucocytic proteases, trypsin, chymotrypsin, and plasminogen activator. Defects in the A1AT gene can lead to emphysema or liver disease. Increased levels of serum A1AT are found in cases of lung and prostate cancers and as an acute phase response to tissue necrosis and inflammation.

Handling

Storage Store at 2 - 8ºC. Do not Freeze.

Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.

Data Sheet