Product Name Anti-Alginate, Pseudomonas-reactive, Antibody (15725)
Description Anti-Alginate Mouse Monoclonal Antibody
Target Alginate, Pseudomonas-reactive,
Species Reactivity Pseudomonas
Clone ID 3G4-1F5
Immunogen Sodium alginate conjugated to KLH
Concentration Lot Specific
Formulation PBS, pH 7.4, 50% glycerol, 0.1% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.1% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Purification Purified by Protein A affinity chromatography
Target ID Alginate, Pseudomonas-reactive,
Research Areas Infectious Disease
Background Pulmonary infection by mucoid, alginate-producing Pseudomonas aeruginosa is the leading cause of mortality among patients suffering from cystic fibrosis. During early childhood, cystic fibrosis patients are colonized by multiple bacterial pathogens including nonmucoid P. aeruginosa. The appearance of mucoid isolates indicates progression to chronic infections. Recent evidence indicates that P. aeruginosa is most resistant to antibiotics when the infecting cells are present as a biofilm, as they appear to be in the lungs of cystic fibrosis patients.
Storage Store at 2 - 8ºC. Do not Freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions ELISA: Use at 0.5-20ug/ml with alginate on the solid phase. Working concentrations for these antibodies are dependent on the purity and concentration of alginate in samples tested.