Anti-PERP Antibody (56225)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC Human
SKU: 56225 Categories: , ,
Overview
Product Name Anti-PERP Antibody (56225)
Description Anti-PERP Rabbit Polyclonal Antibody
Target PERP
Species Reactivity Human
Applications ELISA,WB,ICC
Host Rabbit
Clonality Polyclonal
Immunogen Peptide corresponding to aa 175-193 of human PERP. This sequence differs from that of mouse by three amino acids.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human PERP, a plasma membrane protein involved in the p53 network. p53 binds to the promoter of PERP and transcriptionally activates the PERP gene; the translated PERP protein mediates p53-induced apoptosis leading to cell death.
Target Name p53 apoptosis effector related to PMP-22
Target ID PERP
Uniprot ID Q96FX8
Alternative Names Keratinocyte-associated protein 1, KCP-1, P53-induced protein PIGPC1, Transmembrane protein THW
Gene Name PERP
Sequence Location Cell junction, desmosome, Cell membrane
Biological Function Component of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. {UniProtKB:Q9JK95}.; Plays a role as an effector in the TP53-dependent apoptotic pathway. {UniProtKB:Q9JK95}.
Research Areas Cancer research
Application Images
Description Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 ug of lysates per lane. Antibodies: PERP 56225 (1 ug/mL), PERP, 57-777 (2 ug/mL), beta-actin 3779 (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Description Western Blot Validation in Human Cell Lines
Loading: 15 ug of lysates per lane. Antibodies: PERP 56225 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Description Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide
Loading: 15 ug of lysates per lane. Antibodies: PERP 56225 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Description Immunocytochemistry Validation of PERP in A431 Cells
Immunocytochemical analysis of A431 cells using anti-PEPR antibody (56225) at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1 ug/mL. In immunoblots, a band of 21 kD is detected.

Positive control: A431 cell lysate.
References & Data Sheet