p95/NBS1 (Phospho-Ser343) Polyclonal Antibody
Catalog No.: 43057
Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
Mutations in p95/NBS1 gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.
Antigen: Peptide sequence that includes phosphorylation site of serine 343 (S-L-S(p)-Q-G) derived from human p95/NBS1.
Host Species: Rabbit
Accession no.: O60934, NP_002476.2
Specificity: This antibody detects endogenous human p95/NBS1 only when phosphorylated at serine 343.
Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~95kDa is detected.
These are recommended working dilutions. Enduser should determine optimal dilutions for their application.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Can be stored at 4°C for short-term.
For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.