Catalog No.: 3340
Format: 100ug Protein G-purified antibody in PBS, pH 7.4
p53 is a tumor suppressor protein that is mutated in a wide variety of cancers. p53 is a nuclear protein that mediates cell cycle arrest in response to DNA damage which is important in maintaining genomic stability.
Antigen: Gel-purified p53 containing aa 14-389 of human p53.
Host Species: Mouse
Antibody Class: IgG1
Gene ID: 7157
Accession no.: P04637
This antibody recognizes human, mouse, and dog p53.
ELISA, FACS, IHC (frozen and paraffin), Immunoprecipitation, Immunoblotting.
IHC: use at 2-4ug/ml. Extensive washing may be necessary. Protein digestion and/or microwave antigen retrieval prior to staining is not required.
Immunoprecipitation: use at 10ug/mg of lysate. This antibody reacts only with mutant p53 under non-denaturing conditions.
Immunoblotting: use at 0.5-1.0mg/ml. A band of 53kDa is detected.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20ºC. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.