p53 Monoclonal Antibody
Catalog No.: 3340
Size: 100ug Protein G-purified antibody in PBS, pH 7.4
Antigen: Gel-purified p53 containing aa 14-389 of human p53.
Host Species: Mouse
Antibody Class: IgG1
Preservatives: None. Available on request.
This antibody recognizes human p53, a tumor suppressor protein that is mutated in a wide variety of cancers. p53 is a nuclear protein that mediates cell cycle arrest in response to DNA damage which is important in maintaining genomic stability.
Dilute in PBS or medium which is identical to that used in the assay system.
ELISA, FACS, IHC (frozen and paraffin), Immunoprecipitation, Immunoblotting.
IHC: use at 2-4ug/ml. Extensive washing may be necessary. This product does not require protein digestion or microwave antigen retrieval prior to staining.
IP: use at 10ug/mg lysate. This antibody reacts with only MUTANT p53 protein under non-denaturing conditions.
Immunoblotting: use at 0.5-1.0mg/ml. A band of 53kDa is detected.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Iggo R et al. 1990 Lancet 335: 675-679.
Gannon JV et al. 1990 EMBO 9: 1595-1602.
Said JW et al. 1992 Amer J Path 141: 1343-1348.
Bartok J et al. 1991 Oncogene 6: 1699-1703.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures