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Anti-NKX3.1 (NT) Antibody (34013)

$400.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ELISA Human
SKU: 34013 Categories: , ,
Overview
Product Name Anti-NKX3.1 (NT) Antibody (34013)
Description Anti-NKX3.1 Antibody (NT) Rabbit Polyclonal Antibody
Target NKX3.1 (NT)
Species Reactivity Human
Applications WB,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to aa residues 21-37 (TPSKPLTSFLIQDILRD) of human NKX3.1 conjugated to KLH at the C- terminus.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human NKX3.1 (approx. 26 kDa). This sequence is highly conserved in rat and mouse.
Target Name Homeobox protein Nkx-3.1
Target ID NKX3.1 (NT)
Uniprot ID Q99801
Alternative Names Homeobox protein NK-3 homolog A
Gene Name NKX3-1
Sequence Location Nucleus
Biological Function Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. {PubMed:19462257}.
Research Areas Cancer research
Background NKX3.1 is a homeobox gene which exhibits prostate- and testis-specific expression. Loss of NKX3.1 expression has been implicated in prostate development and tumorigenesis. NKX3.1 expression is dramatically down-regulated in testicular cancer of germ cell origin. Results suggest that NKX3.1 is essential for normal testis function and that its loss of expression is highly associated with the invasive phenotype of testicular germ cell tumors.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting : use at 1:1,000

Positive control: Human prostate tissue extracts.
References & Data Sheet
References Lind GE. et al. (2005) Mol Cancer. 4:8.
Gelmann E.P. et al. (2003) Prostate 55:111-117.