Anti-Neuroligin-4 Antibody (56553)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF Mouse, Rat
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Overview
Product Name Anti-Neuroligin-4 Antibody (56553)
Description Anti-Neuroligin-4 Mouse Monoclonal Antibody
Target Neuroligin-4
Species Reactivity Mouse, Rat
Applications WB,IHC,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID S98-7
Isotype IgG1
Immunogen Fusion protein corresponding to aa 782-945 (intracellular C-terminus) of mouse Neuroligin-4.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.1% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes mouse Neuroligin-4. It does not cross-react with other Neuroligins.
Target Name Neuroligin 4-like
Target ID Neuroligin-4
Uniprot ID B0F2B4
Alternative Names Neuroligin-4, NL-4
Gene Name Nlgn4l
Sequence Location Cell membrane; Single-pass type I membrane protein. Cell junction, synapse, postsynaptic cell membrane. Note=Detected at glycinergic postsynapses in retina. Detected on dendritic spines on cultured neurons.
Biological Function Cell surface protein involved in cell-cell-interactions. Plays a role in the formation or maintenance of synaptic junctions via its interactions (via the extracellular domains) with neurexin family members. Plays a role in synaptic signal transmission. {PubMed:18434543, PubMed:21282647}.
Research Areas Neuroscience
Background Neuroligin-4 is a neuronal cell surface protein and a member of the type-B carboxylesterase- lipase protein family. Members of this family may act as splice site-specific ligands for beta- neurexins and may be involved in the formation and remodeling of central nervous system synapses. Neuroligin-4 interacts with discs large homolog 4 (DLG4). Mutations in this gene have been associated with autism and Asperger syndrome. Alternative splicing results in multiple transcript variants.
Application Images
Description Western Blot analysis of Rat Brain Membrane showing detection of ~125-130 kDa Neuroligin 4  protein using Mouse Anti-Neuroligin 4  Monoclonal Antibody, Clone S98-7 (56553). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 4  Monoclonal Antibody (56553) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~125-130 kDa.
Description Immunohistochemistry analysis using Mouse Anti-Neuroligin 4 Monoclonal Antibody, Clone S98-7 (56553). Tissue: Dentate Gyrus. Species: Mouse. Fixation: formaldehyde-fixed paraffin embedded. Primary Antibody: Mouse Anti-Neuroligin 4 Monoclonal Antibody (56553) at 1:100. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain at 1:1000. Courtesy of: Rachel Reith, NIH/NIMH.
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-2ug/mL. A band of ~125-130kDa is detected.

Immunofluorescence: use at 10ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their application.
References & Data Sheet