Product Name Anti-Sulfotyrosine Mouse Monoclonal Antibody (56623)
Description Anti-Sulfotyrosine Mouse Monoclonal Antibody
Species Reactivity Species Independent
Clone ID 7C5
Immunogen Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
Concentration 1 mg/mL
Formulation PBS pH 7.4, 50% glycerol, 0.09% Sodium azide
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium azide
Buffer Cryopreservative 50% glycerol
Purification Protein G Purified
Background Tyrosine sulfation is a common post-translational protein modification catalyzed by tyrosylprotein sulfotransferase (TPST) (1). Sulfotyrosines mediate interactions between hirudin and thrombin, P-selectin glycoprotein ligand-1 and P-selectin, and von Willebrand factor and factor VIII. Tyrosine sulfation of certain receptors is required for several viruses and parasites to enter their hosts.
Specificity Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.
Target Name Sulfotyrosine
Target ID Sulfotyrosine
Alternative Names Sulfotyrosine, Tyrosine sulfation, Sulfated Tyrosine, S-Tyrosine, sTYR, Sulphotyrosine, Tyrosine Sulphation, Sulphated Tyrosine
PubChem ID 514186
Research Areas Cell Signaling | Post-translational Modifications | Sulfation
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 7C5 . Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Sulfotyrosine Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Description Immunohistochemistry analysis using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 7C5 . Tissue: kidney. Species: Human. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Sulfotyrosine Antibody (D) Composite.
Description Western Blot analysis of Sulfotyrosine-BSA Conjugate showing detection of 67 kDa Sulfotyrosine protein using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 7C5 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Sulfotyrosine-BSA. Lane 3: Tyrosine-BSA. Lane 4: Phosphotyrosine-BSA. Lane 5: BSA. Load: 2 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC (1:25); optimal dilutions for assays should be determined by the user.