Anti-Matallothionein Mouse Monoclonal Antibody (56628)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100 ug WB,ELISA P. aeruginosa
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Overview
Product Name Anti-Matallothionein Mouse Monoclonal Antibody (56628)
Description Anti-Matallothionein Mouse Monoclonal Antibody
Target Metallothionein
Species Reactivity P. aeruginosa
Applications WB,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 1F5
Isotype IgG1
Immunogen Recombinant full length Pseudomonas aeruginosa Metallothionein protein
Properties
Form Liquid
Concentration 1 mg/mL
Formulation PBS pH 7.4, 50% glycerol, 0.09% Sodium azide
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium azide
Buffer Cryopreservative 50% glycerol
Format Purified
Purification Protein G Purified
Specificity Information
Specificity Detects 36 kDa band corresponding to Pseudomonas aeruginosa Metallothionein.
Target Name Metallothionein
Target ID Metallothionein
Uniprot ID Q9I1X5
Alternative Names Metallothionein, CES 1, CES1, Metallothionein 1A, Metallothionein 1S, Metallothionein 2, Metallothionein 2A, Metallothionein IA, Metallothionein II, Metallothionein-1A, Metallothionein-IA, Metallothionein2, MGC32848, MT 1A, MT 2, MT 2A, MT IA, MT II, MT-1A, MT-IA, MT1, MT1A, MT1A_HUMAN, MT1S, MT2, MT2A, MTC, Probable Metallothionein, pmtA
Gene ID 880550
Accession Number NP_250830.1
Research Areas Cell Signaling | Epigenetics and Nuclear Signaling | Transcription | Domain Families | Zinc finger
Application Images
Description Western Blot analysis of Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged showing detection of 36 kDa Metallothionein protein using Mouse Anti-Metallothionein Monoclonal Antibody, Clone 1F5 . Lane 1: Molecular Weight Ladder (MW). Lane 2: GST. Lane 3: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged. Load: 1 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Metallothionein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 36 kDa.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions WB (1:1000); ELISA (1:800); optimal dilutions for assays should be determined by the user.
References & Data Sheet