Anti-Hexanoyl-Lysine adduct Mouse Monoclonal Antibody (56609)

$441.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100 ug WB,ICC/IF,ELISA,FACS,FCM Species Independent
SKU: 56609 Categories: , ,
Overview
Product Name Anti-Hexanoyl-Lysine adduct Mouse Monoclonal Antibody (56609)
Description Anti-Hexanoyl-Lysine adduct Mouse Monoclonal Antibody
Target Hexanoyl-Lysine adduct
Species Reactivity Species Independent
Applications WB,ICC/IF,ELISA,FACS,FCM
Host Mouse
Clonality Monoclonal
Clone ID 5D9
Isotype IgG1
Immunogen Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).
Properties
Form Liquid
Concentration 1 mg/mL
Formulation PBS pH 7.4, 50% glycerol, 0.09% Sodium azide
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium azide
Buffer Cryopreservative 50% glycerol
Format Purified
Purification Protein G Purified
Background Hexanoyl-lysine adduct (HEL) is a lysine adduct of 13-HPODE and is produced by the oxidation of omega-6 polyunsaturated fatty acids (1). It is a biomarker for the initial stage of lipid peroxidation. Lipid peroxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging.
Specificity Information
Specificity Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Name Hexanoyl-Lysine adduct
Target ID Hexanoyl-Lysine adduct
Alternative Names Hexanoyl-Lysine adduct, HEL (Hexanoyl-Lysine adduct), HEL, HEL Adduct, Hexanoyl-Lys adduct, Hexanoyl-Lys, Hexanoyl-Lysine (HEL) adduct, Hexanoyl-Lys (HEL), Hexanoyl Lysine adduct, Hexanoyl-Lysine adduct-modified protein, Hexanoyl-Lysine Adduct (HEL)
Research Areas Cancer | Oxidative Stress | Lipid peroxidation
Application Images
Description Western Blot analysis of Hexanoyl Lysine-BSA Conjugate showing detection of 67 kDa Hexanoyl-Lysine adduct protein using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Hexanoyl Lysine-BSA. Lane 3: BSA. Load: 0.5 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Description Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hexanoyl-Lysine adduct protein using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
Description Flow Cytometry analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9 . Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Description Immunohistochemistry analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9 . Tissue: Heart. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Actin (C) Hexanoyl-Lysine adduct Antibody (D) Composite.
Description Immunohistochemistry analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9 . Tissue: Kidney. Species: Rat. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 10X.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions WB (1:1000); ICC/IF (1:50); ELISA (1:1000); FACS (1:50); FCM (1:50); IHC (1:100); optimal dilutions for assays should be determined by the user.
References & Data Sheet