Methicillin-resistant Staphylococcus aureus (MRSA)

MRSA (Methicillin-resistant Staphylococcus aureus) Monoclonal Antibody

ORDERING INFORMATION

Catalog No.: 15715
Size: 500ug lyophilized purified antibody in PBS at a concentration of 1mg/ml.

BACKGROUND

Methicillin-resistant Staphylococcus aureus (MRSA) survives treatment with broad-spectrum beta-lactam antibiotics including penicillin, methicillin, and cephalosporins. Previously, most MRSA infections occurred in hospitals or other health care settings, such as nursing homes and dialysis centers and were known as “health care-associated MRSA” or HA-MRSA. Older adults and people with weakened immune systems were most at risk of contracting HA- MRSA. More recently, another type of MRSA has occurred among otherwise healthy people in the wider community. This form, “community-associated MRSA”, or CA-MRSA, causes serious skin and soft tissue infections and a potentially lethal form of pneumonia.

SPECIFICATION SUMMARY

Antigen: Gel-purified MRSA protein.
Host Species: Mouse
Antibody Class: IgG1
Preservatives: None

SPECIFICITY

This antibody recognizes the product of the MRSA gene expressed by methicillin-resistant Staphylococcus aureus.

APPLICATIONS

ELISA: use at a 1:5,000 dilution in direct ELISA against purified MRSA protein or MRSA-positive Staphylococcus aureus lysate.
Immunoblotting: use at a 1:2,000 dilution. A band of approx. 70 kDa is detected.
These are recommended dilutions. Enduser should determine optimal dilutions for their applications.

RECONSTITUTION

Reconstitute with distilled H20. Mix gently. Wash the sides of the vial and wait 30-60 seconds before use.

DILUTION INSTRUCTIONS

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE AND STABILITY

This lyophilized product is stable for two years at 4°C. After reconstitution, store aliquots at -20°C.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.