Methicillin-resistant Staphylococcus aureus (MRSA)

 MRSA (Methicillin-Resistant Staphylococcus aureus) Monoclonal Antibody 

ORDERING INFORMATION 

Catalog no. Clone No. MAb Subtype Size 

15715 198 IgG1 500ug 

Format: Lyophilized purified antibody in PBS at a concentration of 1mg/ml. 

BACKGROUND 

Methicillin-resistant Staphylococcus aureus (MRSA) survives treatment with broad-spectrum beta-lactam antibiotics including penicillin, methicillin, and cephalosporins. Previously, most MRSA infections occurred in hospitals or other health care settings, such as nursing homes and dialysis centers and were known as “health care-associated MRSA” or HA-MRSA. Older adults and people with weakened immune systems were most at risk of contracting HA-MRSA. More recently, another type of MRSA has occurred among otherwise healthy people in the wider community. This form, “community-associated MRSA”, or CA-MRSA, causes serious skin and soft tissue infections and a potentially lethal form of pneumonia. 

SPECIFICATION SUMMARY 

Antigen: Gel-purified MRSA protein (penicillin-binding protein 2) from strain EMRSA 16. 

Host Species: Mouse 

Specificity: This antibody recognizes the product of the MRSA gene expressed by methicillin-resistant Staphylococcus aureus. 

APPLICATIONS 

ELISA: use at a 1:5,000 dilution in direct ELISA against purified MRSA protein or MRSA-positive Staphylococcus aureus lysate. 

Immunoblotting: use at a 1:2,000 dilution. A band of approx. 70kDa is detected. 

These are recommended dilutions. Enduser should determine optimal dilutions for their applications. 

RECONSTITUTION / DILUTION INSTRUCTIONS 

Reconstitute to 0.5-1.0mg/ml with pyrogen-free distilled water. Mix gently. Wash the sides of the vial and wait 30-60 seconds before use. Dilute further with PBS. 

STORAGE AND STABILITY 

This lyophilized product is stable for two years at 4°C. After reconstitution, store aliquots at -20°C. 

For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures.