Anti-Influenza A Virus H1N1 Antibody (20303)

$376.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB Influenza A Virus H1N1
SKU: 20303 Categories: , ,
Overview
Product Name Anti-Influenza A Virus H1N1 Antibody (20303)
Description Anti-Influenza A Virus H1N1 Rabbit Polyclonal Antibody
Target Influenza A Virus H1N1
Species Reactivity Influenza A Virus H1N1
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen A/California/14/2009 H1N1 virions
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS containing 0.02% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer Anti-Microbial 0.02% Sodium Azide
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes H1N1 HA (~75kDa), NA (~55kDa), M (~26kDa) and NP (~26-27kDa). M and NP often co- migrate as a single band in SDS-PAGE. It also reacts with A/New Caledonia/20/99, and A/Brisbane/10/2007. Other isolates of H1N1 have not been evaluated.
Target ID Influenza A Virus H1N1
Research Areas Infectious Disease
Background Influenza A viruses are subdivided into 16 H (H1-H16) and 9 N (N1-N9) subtypes. Nomenclature for these virus isolates indicate the influenza virus type (A or B), host species (omitted if human), geographical site, year of isolation, and the H and N subtype (example: A/California/14/2009 H1N1). The main antigenic determinants of Influenza A and B viruses are the hemagglutinin (HA) and neuraminidase (NA) transmembrane glycoproteins. NA forms a tetramer with an average size of 220kDa (~55kDa per monomer). The matrix (M) protein of Influenza A virus is one of the two group-specific internal proteins of the virus. The non-structural protein (NP) exists as a homeodimer (~52kDa) consisting of two identical monomers (each ~26kDa).
Application Images
Description SDS-PAGE and Western blot analysis of 3ug intact A/California/14/2009 H1N1 virions. Influenza A virus polyclonal antibody was diluted 1:1,000.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500-1:1,000 dilution

These are recommended dilutions.User should determine optimal dilutions for their application.
References & Data Sheet