Anti-IL-1RAcP Antibody (2129)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human, Mouse, Rat
SKU: 2129 Categories: , ,
Overview
Product Name Anti-IL-1RAcP Antibody (2129)
Description Anti-IL-1RAcP (CT) Rabbit Polyclonal Antibody
Target IL-1RAcP
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 525-540 of human IL-1RAcP. The sequence is identical to that of mouse and rat IL-1RAcP.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat IL-1RAcP (66 kD). No cross-reactivity with other members of the IL-1 receptor family.
Target Name Interleukin-1 receptor accessory protein
Target ID IL-1RAcP
Uniprot ID Q9NPH3
Alternative Names IL-1 receptor accessory protein, IL-1RAcP, EC 3.2.2.6, Interleukin-1 receptor 3, IL-1R-3, IL-1R3
Gene Name IL1RAP
Gene ID 3556
Accession Number NP_001161401
Sequence Location [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.; [Isoform 3]: Secreted.
Biological Function Coreceptor for IL1RL2 in the IL-36 signaling system (By similarity). Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 dUniProtKB:Q61730, PubMed:10799889, PubMed:9371760, PubMed:10653850, PubMed:19836339}.; [Isoform 2]: Associates with secreted ligand-bound IL1R2 and increases the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors (PubMed:12530978). Enhances the ability of secreted IL1R1 to inhibit IL-33 signaling (By similarity). {UniProtKB:Q61730, PubMed:12530978}.; [Isoform 4]: Unable to mediate canonical IL-1 signaling (PubMed:19481478). Required for Src phosphorylation by IL1B. May be involved in IL1B-potentiated NMDA-induced calcium influx in neurons (By similarity). {UniProtKB:Q61730, PubMed:19481478}.
Research Areas Neuroscience
Background Cellular responses induced by the pro- inflammatory cytokine IL-1 require two subunits of the IL-1 receptor, IL-1 receptor 1 (IL-1R1) and IL-1 receptor accessory protein (IL-1RacP). IL- 1RacP forms a complex with IL-1R1 in response to IL-1 treatment. IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kappaB inducing kinase (NIK) and NF-kappaB and associates with the IL-1Rcomplex through IL- 1RacP. IL-1 activation of stress- activated protein kinase andof acid sphingomyelinase also requires IL- 1RacP. Like IL-1R1, the IL-1RacP subunit is essential for IL-1 mediated cellular responses. IL-1RacP is expressed in a variety of tissues.
Application Images
Description Western blot analysis of IL-1RAcP in HeLa whole cell lysate with IL-1RAcP antibody at 1 ug/mL.
Description Immunocytochemistry of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 2 ug/ml.
Description Immunofluorescence of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 5 ug/mL. Red: IL-1RAcP Antibody (2129)
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:1,000-1:2,000 dilution.

Positive control: Whole cell lysate from HeLa cells.
References & Data Sheet