Catalog No.: 2129
Size: 100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chroma- tography.
Cellular responses induced by the pro- inflammatory cytokine IL-1 require two subunits of the IL-1 receptor, IL-1 receptor 1 (IL-1R1) and IL-1 receptor accessory protein (IL-1RacP). IL- 1RacP forms a complex with IL-1R1 in response to IL-1 treatment. IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB inducing kinase (NIK) and NF-κB and associates with the IL-1Rcomplex through IL- 1RacP. IL-1 activation of stress- activated protein kinase andof acid sphingomyelinase also requires IL- 1RacP. Like IL-1R1, the IL-1RacP subunit is essential for IL-1 mediated cellular responses. IL-1RacP is expressed in a variety of tissues.
Antigen: Peptide corresponding to aa 525-540 of human IL-1RAcP. The sequence is identical to that of mouse and rat IL-1RAcP.
Host Species: Rabbit
Preservatives: 0.02% sodium azide.
This antibody recognizes human, mouse, and rat IL-1RAcP (66 kD). No cross-reactivity with other members of the IL-1 receptor family.
Immunoblotting: use at 1:1,000-1:2,000 dilution.
Positive control: Whole cell lysate from HeLa cells.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.