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Anti-Hsp40 (Hdj1) Antibody (11111)

$384.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA,IHC,IP Human, Mouse, Rat
SKU: 11111 Categories: , ,
Overview
Product Name Anti-Hsp40 (Hdj1) Antibody (11111)
Description Anti-Hsp40 Mouse Monoclonal Antibody
Target Hsp40 (Hdj1)
Species Reactivity Human, Mouse, Rat
Applications WB,ELISA,IHC,IP
Host Mouse
Clonality Monoclonal
Clone ID 3B9.E6
Isotype IgG1
Immunogen Recombinant Hsp40 (Hdj1)
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat Hsp40 (Hdj1). Other species have not been tested. It does not cross-react with Hdj2 or Ydj1.
Target Name DnaJ homolog subfamily B member 1
Target ID Hsp40 (Hdj1)
Uniprot ID P25685
Alternative Names DnaJ protein homolog 1, Heat shock 40 kDa protein 1, HSP40, Heat shock protein 40, Human DnaJ protein 1, hDj-1
Gene Name DNAJB1
Gene ID 3337
Accession Number NP_006136.1
Sequence Location Cytoplasm, Nucleus, Nucleus, nucleolus
Biological Function Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and rPubMed:24318877, PubMed:9499401}.
Research Areas Heat Shock& Stress Proteins
Background Members of the human Hsp40/DnaJ protein family feature a J domain (named for the bacterial DnaJ protein). The J domain spans the first 75 N- terminal amino acids and is separated from the C-terminus by a glycine- phenylalanine-rech domain. The Hsp40/DnaJ proteins play many different roles in cellular processes such as folding, translocation, degradation, and assembly of multi-protein complexes. Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding as well as in regulation of Hsp70 function. Hsp40 stimulates ATPase activity of Hsp70 which in turn causes conformational changes in unfolded proteins.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1ug/ml. A band of ~40 kDa is detected.ELISA

User should determine optimal concentrations for their application.

Positive control: HeLa cell lysate.
References & Data Sheet