Anti-HNF4α (phospho-Ser304) Antibody (43043)
$355.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Rabbit | 100ul | WB | Human, Mouse, Rat |  |
Overview
Product Name Anti-HNF4α (phospho-Ser304) Antibody (43043)
Description Anti-HNF4α (phospho-Ser304) Rabbit Polyclonal Antibody
Target HNF4α (phospho-Ser304)
Species Reactivity Human, Mouse, Rat
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of Serine 304 (L-R-S(p)-Q-V) derived from human HNF4a and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human, mouse, and rat HNF4alpha only when phosphorylated at serine 304.
Target Name Hepatocyte nuclear factor 4-α
Target ID HNF4α (phospho-Ser304)
Uniprot ID P41235
Gene Name HNF4A
Gene ID 600281
Post Translational Modification HNF4α (phospho-Ser304)
Target Phospho-ser304
Sequence Location Nucleus.
Biological Function Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes (PubMed:30597922). Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells (PubMed:30530698). {UniProtKB:P49698, PubMed:30530698, PubMed:30597922}.
Research Areas Phosphospecific Antibodies
Background Hepatocyte nuclear factor 4 alpha (HNF4alpha) is a nuclear transcription factor that binds DNA as a homodimer and controls the expression of several genes including hepatocyte nuclear factor 1, a transcripton factor that regulates expression of several hepatic genes. HNF4alpha has been implicated in development of liver, kidney, and intestines. Mutations in the HNF4alpha gene have been associated with monogenic autosomal-dominant non-insulin-dependent diabetes mellitus type 1. Alternative splicing of this gene results in multiple transcript variants.
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:1,000. A band of ~52kDa is detected.
These are recommended working dilutions.
End user should determine optimal dilutions for their applications
These are recommended working dilutions.
End user should determine optimal dilutions for their applications
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet