GluA1/GluR1 Monoclonal Antibody
Catalog No.: 56547 (clone S355-1)
Size: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Regulation of α-amino-3-hydroxy-5- methyl-4-isoxazole propionic acid receptors (AMPARs) plays a key role in altering excitatory synaptic transmission in the CNS. Interestingly, the regulatory mechanisms differ between distinct subunits of AMPAR, which range from glutamate receptor 1 (GluR1) to GluR4 (also referred to as GluA1–4). For example, subunits with a long intracellular carboxy terminus (i.e., GluR1, GluR2L, and GluR4) are involved in activity- dependent synaptic targeting of AMPAR, whereas those with a shorter carboxy terminus (i.e., GluR2, GluR3, and GluR4s) seem to maintain basal synaptic transmission. GluR1 has several phosphorylation sites on the intracellular carboxy terminus. Many of these sites have been demonstrated to play a role in synaptic AMPAR regulation and synaptic plasticity.
Antigen: Fusion protein corresponding to aa 1-389 (extracellular N-terminus) of rat GluA1/GluR1 (accession no. P19490).
Host Species: Mouse
Antibody Class: IgG1
This antibody recognizes mouse and rat GluA1/GluR1. It does not cross-react with GluR2.
Immunoblotting: use at 1ug/ml. A band of ~100kDa is detected.
Positive control: Rat brain lysate.
These are recommended concentrations. User should determine optimal concentrations for their application.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.