FKHR / FOX01 Monoclonal Antibody
Catalog No.: 3338
Clone No.: 7H3
Format: 100ug Protein G-purified antibody in PBS, pH 7.4.
FKHR (ForKHead in Rhabdomyosarcoma), a 70kDa protein, is a member of the forkhead family of transcription factors. Members of this family are classified by their degree of homology in the 100-120aa region that is necessary for DNA binding and binding-site specificity. The FKHR subfamily differs from other members of the forkhead family in that all members contain an insertion of 5 amino acids next to the helix 3 in the middle of the forkhead domain and lack the conserved amino terminal KPPY sequence found in most forkhead genes. It has been shown that members of the FKHR gene family are phosphorylated by Akt which regulates its cellular localization promoting cell survival.
Antigen: GST fusion protein containing the region encoding aa 242-655 of human FKHR expressed in E. coli.
Host Species: Mouse
Antibody Subtype: IgG1
Specificity: This antibody recognizes human FKHR/FOX01.
Immunoblotting: use at 0.1-2.0ug/ml. A band of 70kDa is detected.
Endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C or below. Store undiluted product in appropriate aliquots to avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures.