Anti-Fibroblast Growth Factor Receptor 1 (FGFR1) Antibody (30103)

Host	Quantity	Applications	Species Reactivity	Data Sheet
Mouse	250ug	WB,IHC,IP	Human

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SKU: 30103 Categories: Antibody Products, Growth Factors/Cytokines/Receptor Antibodies, Products

Overview

Product Name Anti-Fibroblast Growth Factor Receptor 1 (FGFR1) Antibody (30103)

Description Anti-FGF Receptor 1 Mouse Monoclonal Antibody

Target Fibroblast Growth Factor Receptor 1 (FGFR1)

Species Reactivity Human

Applications WB,IHC,IP

Host Mouse

Clonality Monoclonal

Clone ID M17D10

Isotype IgG1

Immunogen Recombinant human ectodomain of FGFr1b expressed in E. coli from pro23 to his415; antigen contained NH2-terminal gly-ser-pro-gly- ile and COOH-terminal glu-phe sequences.

Properties

Form Liquid

Concentration Lot Specific

Formulation PBS, pH 7.4

Buffer Formulation Phosphate Buffered Saline

Buffer pH pH 7.4

Format Purified

Purification Purified by Protein G affinity chromatography

Specificity 30103 reacts with both alpha and b isoforms. Epitope is within the sequence pro179 to lys195 in Ig loop II.

Target Name Fibroblast growth factor receptor 1

Target ID Fibroblast Growth Factor Receptor 1 (FGFR1)

Uniprot ID P11362

Alternative Names FGFR-1, EC 2.7.10.1, Basic fibroblast growth factor receptor 1, BFGFR, bFGF-R-1, Fms-like tyrosine kinase 2, FLT-2, N-sam, Proto-oncogene c-Fgr, CD antigen CD331

Gene Name FGFR1

Sequence Location Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle. Note=After ligand binding, both receptor and ligand are rapidly internalized. Can translocate to the nucleus after internalization, or by translocation from the endoplasmic reticulum or Golgi apparatus to the cytosol, and from there to the nucleus.

Biological Function Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation. {UniProtKB:P16092, PubMed:10830168, PubMed:11353842, PubMed:12181353, PubMed:1379697, PubMed:1379698, PubMed:15117958, PubMed:16597617, PubMed:17311277, PubMed:17623664, PubMed:18480409, PubMed:19224897, PubMed:19261810, PubMed:19665973, PubMed:20133753, PubMed:20139426, PubMed:21765395, PubMed:8622701, PubMed:8663044}.

Research Areas Growth Factors, Cytokines, Receptors

Handling

Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.

Application Instructions Western blotting: at 1 ug/ml recognizes 10 ng recombinant bacterial, baculo- viral, or native FGFr1.

Immunoprecipitation: native, recombinant, and ligand-labeled FGFr1.

Immunohistochemistry: under investigation.

References Xu et al. (1992) J Biol Chem 267: 1779
Robinson et al. (1995) Development 121: 3959.

PMID 8575296

Data Sheet