Epidermal Growth Factor-like Protein 7 Monoclonal Antibody
Catalog No.: 90013 (clone 2H2)
Format: 100ug, Protein A-purified, lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
EGF-L7 (Epidermal Growth Factor Like Protein 7) is a multi-domain protein containing two EGF like domains and one EMI domain. It is expressed almost exclusively in endothelial cells and functions to promote normal development of the vascular system. EGF-L7 is capable of antagonistic binding to Notch receptors resulting in the inhibition of Notch signaling in HUVEC and neural stem cells. In research models inducing hypoxia and subsequent reoxygenation, (H/R), EGF- L7 can inhibit ICAM-1 expression and enhance the inhibition of NF-kappaB activation. Additionally, EGF-L7 is a chemoattractant for endothelial cells and binds to the extracellular matrix. The overexpression of EGF-L7 is observed in various cancers, and is generally correlated with increased metastasis and a poor prognosis.
Antigen: His-tagged EGFL-7 recombinant protein. Accession no. Q9UHF1.
Host species: Mouse.
Antibody Class: IgG1.
This antibody reacts with human EGFL- 7 protein.
ELISA: use at 1-10ug/ml.
Immunoblotting: use at 1-5ug/ml. A band of ~30kDa is detected.
Immunohistochemistry: Use at 2- 10ug/ml.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Reconstitute in distilled water.
STORAGE AND STABILITY
This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.