Endotoxin

Gram-Negative Endotoxin Monoclonal Antibodies

ORDERING INFORMATION

Catalog No. 15301	Clone No. GNE13-337.5	MAb Subtype IgG2b*	Size 100ug, 500ug	Library Pack No. 153101	100ug/clone All 9 clones
15302	GNE13-305.6	IgG2a*	100ug, 500ug
15303	GNE10-113.3	IgG3**	100ug, 500ug
15304	GNE11-265.5	IgG3**	100ug, 500ug
15305	GNE11-270.3.1	IgG2a*	100ug, 500ug
15306	GNE11-222.4	IgM**	100ug, 500ug
15307	HEC10-172.5	IgM**	100ug, 500ug
15308	HEC11-445.2	IgM**	100ug, 500ug
15309	IVEC9-220.1	IgM**	100ug, 500ug

Format: *Protein G-purified antibody in PBS, pH 7.4
**PEG-purified antibody in PBS, pH 7.4

BACKGROUND

Endotoxin is the lipopolysaccharide (LPS) complex associated with the outer membrane of Gram- negative bacteria such as Escherichia coli, Salmonella, Shigella, Pseudomonas, Neisseria, Haemophilus influenzae, Bordetella pertussis and Vibrio cholerae. Toxicity is associated with the lipid component (Lipid A) and immunogenicity is associated with the polysaccharide components. The cell wall antigens (O antigens) of Gram-negative bacteria are components of LPS. LPS elicits a variety of inflammatory responses, and it activates complement by the alternative (properdin) pathway.

SPECIFICATION SUMMARY

Antigen: E. coli O:111 B4 J5 cells
Host Species: Mouse

Host Species:	Mouse
Catalog No.	Reactivity	Reactivity Key
15301	2, 3, 4, 5	2. E. coli J5 LPS
15302	2,4,5	3. Klebsiella pneumoniae
15303	2	4. Shigella sonnei
15304	2,4	5. Salmonella typhimurium
15305	2, 3, 4, 5	6. Enterobacter aerogenes
15306	2, 4, 5, 7, 8, 9, 10	7. Serratia marcescens
15307	2, 3, 7, 8, 9, 11	8. Proteus mirabilis
15308	2, 6, 7, 8, 10, 11	9. Proteus vulgaris
15309	2, 3, 6, 7, 8, 9, 10, 11	10. Acinetobacter calcoaceticus
		11. Pseudomonas aeruginosa

APPLICATIONS

These antibodies have been qualified for use in ELISA to detect gram-negative endotoxin and in dot-blots to detect cells that express gram negative endotoxin. Endusers should determine optimal concentrations for their applications.
Customer feedback: #15305 was conjugated to colloidal gold and used successfully in a lateral flow assay format.

DILUTION INSTRUCTIONS

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE AND STABILITY

These antibodies are stable for at least one (1) year at -20o to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures.

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