Anti-Elastase, Neutrophil Antibody (13204)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug
1mg
500ug 		ELISA,Neutralization Human
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SKU: 13204 Categories: , ,
Overview
Product Name Anti-Elastase, Neutrophil Antibody (13204)
Description Anti-Elastase (Neutrophil) Mouse Monoclonal Antibody
Target Elastase, Neutrophil,
Species Reactivity Human
Applications ELISA,Neutralization
Host Mouse
Clonality Monoclonal
Clone ID ELA10-103.2
Isotype IgG2b
Immunogen Purified human neutrophil elastase.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH 7.4
Format Purified
Purification Purified by protein G affinity chromatography
Specificity Information
Target Name Neutrophil elastase
Target ID Elastase, Neutrophil,
Uniprot ID P08246
Alternative Names EC 3.4.21.37, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, HLE, Medullasin, PMN elastase
Gene Name ELANE
Sequence Location Cytoplasmic vesicle, phagosome
Biological Function Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984). {PubMed:10947984, PubMed:15140022}.
Research Areas Enzymes
Application Images
Description These antibodies recognize human neutrophil elastase and vary in their ability to inhibit neutrophil elastase activity as determined in an assay of elastase-mediated hydrolysis of elastin-Congo Red substrate:
Additional Information
Handling
Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions These antibodies have been qualified for use in ELISA to detect human neutrophil elastase and in studies of inhibition and enzyme kinetics of human neutrophil elastase.
References & Data Sheet