Elastase, Neutrophil

Neutrophil Elastase Monoclonal Antibodies

ORDERING INFORMATION

Catalog No.	Clone No.	MAb Subtype	Size	Library Pack No.	100ug/clone
13201	ELA11-207.2	IgM1	100ug, 500ug	132100	All 4 clones
13202	ELA11-207.3	IgM1	100ug, 500ug
13203	ELA10-101.5	IgG12	100ug, 500ug
13204	ELA10-103.2	IgG2b2	100ug, 500ug

BACKGROUND

Neutrophil elastase is a major neutral protease present in human neutrophils and is released by neutrophils following stimulation with a variety of stimuli. It is believed to be a major mediator of the lung destruction seen in emphysema. In addition to elastin, neutrophil elastase can cleave other biologically active proteins such as fibrinogen and antithrombin and is released by neutrophils during clot formation.

SPECIFICATION SUMMARY

Antigen: Purified human neutrophil elastase.
Host Species: Mouse
Specificity: These antibodies recognize human neutrophil elastase and vary in their ability to inhibit neutrophil elastase activity as determined in an assay of elastase-mediated hydrolysis of elastin-Congo Red substrate:

Catalog No. 13201	% Elastase Inhibition 1.2
13202	4.1
13203	90.0
13204	82.0

APPLICATIONS

These antibodies have been qualified for use in ELISA to detect human neutrophil elastase and in studies of inhibition and enzyme kinetics of human neutrophil elastase.

DILUTION INSTRUCTIONS

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE AND STABILITY

These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures.

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