Anti-E2F-1 Antibody (3332)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC-P,IP Human
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SKU: 3332 Categories: , ,
Overview
Product Name Anti-E2F-1 Antibody (3332)
Description Anti-E2F-1 Mouse Monoclonal Antibody
Target E2F-1
Species Reactivity Human
Applications WB,IHC-P,IP
Host Mouse
Clonality Monoclonal
Clone ID 2F10
Isotype IgG1
Immunogen GST fusion protein expressed in E. coli corresponding to aa 162-476 of human E2F-1.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes an epitope within aa 332-390 of human E2F-1.
Target Name Transcription factor E2F1
Target ID E2F-1
Uniprot ID Q01094
Alternative Names E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1
Gene Name E2F1
Gene ID 1869
Accession Number NP_005216
Sequence Location Nucleus
Biological Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 rPubMed:10675335, PubMed:12717439, PubMed:17050006, PubMed:17704056, PubMed:18625225, PubMed:20040599, PubMed:20176812, PubMed:28992046, PubMed:8170954}.
Research Areas Cancer Research
Application Images
Description Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [17E2] (3332) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Description E2F1 antibody [17E2] detects E2F1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
E2F1 stained by E2F1 antibody [17E2] (3332) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-5 ug/mL.

Immunoprecipitation: use at 1-5 ug/mL.

Positive controls: E2F-1 transfected Soas2 cells and recombinant fusion protein.
References & Data Sheet