Dicer

Dicer Monoclonal Antibody

ORDERING INFORMATION

Catalog No.: 12532 (clone S167-7)
Size: 100ug in PBS (1mg/ml), pH 7.4, 50% glycerol, 0.09% sodium azide.
Purified by Protein G affinity chromatography.

BACKGROUND

Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs). After long primary transcript pri-miRNAs are processed to stem-looped pre- miRNAs by Drosha, pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22- nucleotide mature miRNAs . The mature miRNA then becomes a part of the RNA- Induced Silencing Complex (RISC) and can bind to the 3′ UTR of the target mRNA.

SPECIFICATION SUMMARY

Antigen: Fusion protein corresponding to aa 1638-1899 of mouse Endoribonuclease Dicer (accession no.Q8R418).
Host Species: Mouse
Antibody Class: IgG1

SPECIFICITY

This antibody recognizes human, mouse and rat Dicer.

APPLICATIONS

Immunoblotting: use at 1ug/ml. Predicted molecular weight is ~215kDa.
Positive Control: rat brain lysate.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

DILUTION INSTRUCTIONS

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE AND STABILITY

This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze- thaw cycles.

For in vitro investigational use only. Not intended for diagnostic or therapeutic applications.

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