Anti-Chk2 (NT) Antibody (56221)

$469.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human, Mouse, Rat
SKU: 56221 Categories: , ,
Overview
Product Name Anti-Chk2 (NT) Antibody (56221)
Description Anti-Chk2 (NT) Rabbit Polyclonal Antibody
Target Chk2 (NT)
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 2-18 of human Chk2.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes an epitope at the N-terminus of human, mouse, and rat Chk2 (Checkpoint Kinase 2). In response to DNA damage, ATM phosphorylates and activates Chk2 which in turn directly phosphorylates and activates p53. Chk2 also phos- phorylates and activates BRCA1, the product of a tumor suppressor gene that is often mutated in breast and ovarian cancer.
Target Name Serine/threonine-protein kinase Chk2
Target ID Chk2 (NT)
Uniprot ID O96017
Alternative Names EC 2.7.11.1, CHK2 checkpoint homolog, Cds1 homolog, Hucds1, hCds1, Checkpoint kinase 2
Gene Name CHEK2
Sequence Location [Isoform 2]: Nucleus. Note=Isoform 10 is present throughout the cell.; [Isoform 4]: Nucleus.; [Isoform 7]: Nucleus.; [Isoform 9]: Nucleus.; [Isoform 12]: Nucleus.; Nucleus, PML body. Nucleus, nucleoplasm. Note=Recruited into PML bodies together with TP53.
Biological Function Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous rUniProtKB:Q9Z265, PubMed:10097108, PubMed:10724175, PubMed:11298456, PubMed:12402044, PubMed:12607004, PubMed:12717439, PubMed:12810724, PubMed:16163388, PubMed:17101782, PubMed:17380128, PubMed:17715138, PubMed:18317453, PubMed:18644861, PubMed:18728393, PubMed:20364141, PubMed:25361978, PubMed:25619829, PubMed:9836640, PubMed:9889122}.; (Microbial infection) Phosphorylates herpes simplex virus 1/HHV-1 protein ICP0 and thus activates its SUMO-targeted ubiquitin ligase activity. {PubMed:32001251}.
Research Areas Cancer research
Application Images
Description Western blot analysis of Chk2 expression in (A) K562, (B) Jurkat, and (C) HL-60 whole cell lysates with Chk2 antibody at 1 ug/ml.
Description Immunocytochemistry of Chk2 in Jurkat cells with Chk2 antibody at 1 ug/mL.
Description Immunofluorescence of Chk2 in Jurkat cells with Chk2 antibody at 5 ug/mL Red: Chk2 Antibody (56221)
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1 ug/mL. In immunoblots, a band of 60 kD is detected.

Positive control: Jurkat cell lysate.
References & Data Sheet