Anti-CDX-2 Antibody

$243.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ul IHC Human
SKU: 34055 Categories: , , Tag:
Overview
Product Name Anti-CDX-2 Antibody (34055)
Description Anti-CDX-2 Mouse Monoclonal Antibody
Target CDX-2
Species Reactivity Human
Applications IHC
Host Mouse
Clonality Monoclonal
Clone ID G402.1
Isotype IgG2a
Immunogen Recombinant human CDX-2.
Properties
Form Liquid
Concentration Lot Specific
Formulation Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.
Buffer Formulation Tris
Buffer pH pH 7.3-7.7
Buffer Anti-Microbial 0.1% Sodium Azide
Buffer Protein Stabilizer 1% Bovine Serum Albumin
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity Human CDX-2. Reactivity with other species has not been investigated.
Target Name Homeobox protein CDX-2
Target ID CDX-2
Uniprot ID Q99626
Alternative Names CDX-3, Caudal-type homeobox protein 2
Gene Name CDX2
Sequence Location Nucleus
Biological Function Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium (By similarity). Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription (By similarity). Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription (By similarity). Binds to and activates transcription of LPH (By similarity). Activates transcription of CLDN2 and intestinal mucin MUC2 (By similarity). Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription (By similarity). Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA (PubMed:28473536). {UniProtKB:P43241, UniProtKB:Q04649, PubMed:28473536}.
Research Areas Cancer research
Background Homeobox protein CDX-2 is a protein that in humans is encoded by the CDX2 gene. This gene is a member of the caudal-related homeobox transcription factor family that is expressed in the nuclei of intestinal epithelial cells, and, as such, is a useful marker for gastrointestinal carcinoma as well as for determining the origin of gastrointestinal metastatic adenocarcinoma and carcinoids.
Application Images
Description Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes. Detection of CDX-2 in human colon with #34055 diluted 1:100-1:200.
Additional Information
Handling
Storage Store at 2 - 8ºC. Do not Freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions

Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.
References & Data Sheet