Anti-CD4 Cytoplasmic Tail Antibody (18715)

Host	Quantity	Applications	Species Reactivity	Data Sheet
Mouse	100ug	WB,ELISA,IF	Human

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SKU: 18715 Categories: Antibody Products, Cell Adhesion Molecule Antibodies, Products

Overview

Product Name Anti-CD4 Cytoplasmic Tail Antibody (18715)

Description Anti-CD4 Cytoplasmic Tail Mouse Monoclonal Antibody

Target CD4 Cytoplasmic Tail

Species Reactivity Human

Applications WB,ELISA,IF

Host Mouse

Clonality Monoclonal

Clone ID 10C12

Isotype IgG1

Immunogen Synthetic peptide corresponding to aa 439-458 (LSEKKTCQCPHRFQKTCSPI)

Properties

Form Liquid

Concentration Lot Specific

Formulation PBS, pH 7.4.

Buffer Formulation Phosphate Buffered Saline

Buffer pH pH 7.4

Format Purified

Purification Purified by Protein G affinity chromatography

Specificity This antibody recognizes an epitope within the region of amino acids 439-458 of the cytoplasmic tail region of human CD4.

Target Name T-cell surface glycoprotein CD4

Target ID CD4 Cytoplasmic Tail

Uniprot ID P01730

Alternative Names T-cell surface antigen T4/Leu-3, CD antigen CD4

Gene Name CD4

Accession Number NP_000607

Sequence Location Cell membrane

Biological Function Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. {PubMed:16951326, PubMed:24942581, PubMed:2823150, PubMed:7604010}.; (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed:2214026, PubMed:16331979, PubMed:9641677, PubMed:12089508). Down-regulated by HIV-1 Vpu (PubMed:17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed:7909607). {PubMed:12089508, PubMed:16331979, PubMed:17346169, PubMed:2214026, PubMed:7909607, PubMed:9641677}.

Research Areas Cell adhesion

Background CD4 is a co-receptor of the T-cell receptor (TCR) and assists the TCR in communicating with antigen-presenting cells. The short cytoplasmic/intracellular tail of CD4 contains a special sequence of amino acids that allow it to recruit and interact with the tyrosine kinase Lck (lymphocyte-specific protein tyrosine kinase). When the TCR and CD4 bind to distinct regions of an antigen-presenting MHCII molecule, tyrosine kinase Lck bound to the cytoplasmic tail of CD4 tyrosine-phosphorylates immunoreceptor tyrosine activation motifs (ITAM) on the cytoplasmic domains of CD3 to amplify the signal generated by the TCR. Many functions have been assigned to the cytoplasmic tail of CD4 including anti-viral regulation (down/up regulation of CD4), T-cell maturation, activation and differentiation, and Lck signal transmission.

Handling

Storage This antibody is stable for at least one (1) year at -20°C.

Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.

Application Instructions ELISA: use at 10-20ug/ml. Optimized with 25ug cell lysate (Jurkat, H9, HeLa) on the solid phase.

Data Sheet