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Campylobacter IgA / recomLine

$550.00

Host Quantity Applications Species Reactivity Data Sheet  
20 Tests Lateral Strip Assay Campylobacter jejuni, Campylobacter coli
SKU: 6273 Categories: ,
Overview
Product Name recomLine Campylobacter IgA Lateral Strip Test Kit (6273)
Description Strip-Immunoassay with antigens produced by recombinant techniques for the detection of IgG and IgA antibodies against Campylobacter jejuni and Campylobacter coli
Target Campylobacter IgA
Product Background The genus Campylobacter comprises gram-negative, spiral-shaped, microaerophilic, mesophilic to thermophilic bacteria with bipolar flagella. Human Campylobacter infections are mainly food associated intestinal infections with world-wide incidence. Contaminated and insufficient cooked foods or drinking water in tropical countries are the main sources of infection. The pathogen reservoir is mainly the intestinal tract of warm-blooded wild, domestic and pet animals. Intestinal Campylobacter infections are the second most frequent enteric bacterial infections reported in Germany after enteric Salmonelloses, whereas unreported cases not reflected in the statistics probably outnumber reported cases many times over.Campylobacter jejuni is much more frequent, accounting for over 90% of cases as compared to Campylobacter coli at approx. 9%. Besides nearly asymptomatic (clinically inapparent) courses, infected persons suffer from painful gastrointestinal symptoms with sometimes bloody diarrhoea, fever, meningism and myalgias. In rare cases sequelae like postinfectious Reactive Arthritis or Guillain-Barré Syndrome may develop with onset a few weeks after the primary infection.recomLine Campylobacter is a qualitative in-vitro test for the detection of IgG and IgA anti­bodies against immunodominant antigens of Campylobacter jejuni and Campylobacter coli. In cases of previous or persistent Campylobacter infection with primary diagnosis based on stool sample culturing, recomLine Campylobacter provides for identification of specific Campylobacter antibodies by means of separate lineups of different antigens produced by recombinant engineering for the purpose of clarifying postinfection complications.
Species Reactivity Campylobacter jejuni, Campylobacter coli
Applications Lateral Strip Assay
Properties
Specificity Information
Target ID Campylobacter IgA
Research Areas Infectious Disease
Application Images
Description Strip layout.
Description Test principle and procedure:

1. 1st Incubation. A test strip loaded with Campylobacter antigens is incubated with diluted serum or plasma in a dish for 1 hour.
2. Wash 3 times
3. 2nd Incubation
4. Peroxidase conjugated anti-human antibodies (IgG or IgA specific) are added. Incubate for 45 minutes.
5. Wash 3 times
6. Color reaction. 5 - 10 minutes after addition of the coloring solution, insoluble colored bands develop at the sites on the test strips occupied by antibodies.
Description Related recombinant Campylobacter antigens
Description Test evaluation.

Patient samples from different sources were tested to assess the performance capability of recomLine Campylobacter IgG IgA. Additional data on the frequency of positive reactivity for the individual recombinant antigens are listed.

* Samples from patients with positive Campylobacter stool cultures (C. jejuni, C. coli). Blood samples were taken 0 to 40 days after the stool diagnostics, the beginning of the disease was unknown.
** Samples from patients with suspected Guillain-Barré-Syndrom (GBS) unclarified aetiology. No data on previous illnesses involving diarrhoea were known. (Testet with recomLine Campylobacter without P39)
*** Samples from patients with suspected Reactive Arthritis (ReA) unclarified aetiology. No data on previous illnesses involving diarrhoea were known.
Additional Information
Components
10X Wash Buffer100 mL
TMB Substrate40 mL
Milk Powder5 g
Instuctions for Use1 Each
Evaluation Form1 Each
Test Strips2 Vials x 10 Each
Anti-Human IgA Conjugate500 uL
Handling
Storage Store at 2 - 8ºC. Do not Freeze.
References & Data Sheet