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Anti-c-myc Antibody

$406.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,IP,ICC,ELISA C-Myc
SKU: 18826-01 Categories: , ,
Overview
Product Name Anti-c-myc Antibody (18826-01)
Description Anti-c-myc Rabbit Polyclonal Antibody
Target c-myc
Species Reactivity C-Myc
Applications WB,IP,ICC,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen Amino acids 410-419 (EQKLISEEDL) of human myc conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.2, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Target ID c-myc
Research Areas Epitope Tags
Background c-myc is an epitope tag often constructed on the N- or C-terminus of a protein to allow for detection and analysis of the protein. The peptide sequence of the myc tag is EQKLISEEDL (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu). The c-myc tag is approximately 1202 Daltons in atomic mass and includes 10 amino acids.
Application Images
Additional Information
Handling
Storage Store at 2 - 8ºC. Do not Freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000- 1:25,000

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunofluorescence: use at a dilution of 1:100-1:400

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500.

These are recommended dilutions.

End users should determine optimal dilutions for their applications.
References & Data Sheet