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Anti-c-myc Antibody (18826-01)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,IP,ICC,ELISA C-Myc
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SKU: 18826-01 Categories: , ,
Overview
Product Name Anti-c-myc Antibody (18826-01)
Description Anti-c-myc Rabbit Polyclonal Antibody
Target c-myc
Species Reactivity C-Myc
Applications WB,IP,ICC,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen Amino acids 410-419 (EQKLISEEDL) of human myc conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.2, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by peptide affinity chromatography
Specificity Information
Target ID c-myc
Research Areas Epitope Tags
Background c-myc is an epitope tag often constructed on the N- or C-terminus of a protein to allow for detection and analysis of the protein. The peptide sequence of the myc tag is EQKLISEEDL (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu). The c-myc tag is approximately 1202 Daltons in atomic mass and includes 10 amino acids.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000- 1:25,000

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunofluorescence: use at a dilution of 1:100-1:400

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500.

These are recommended dilutions.

End users should determine optimal dilutions for their applications.
References & Data Sheet