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Anti-BRCAA1 Antibody (34022)

$376.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB Human, Mouse, Rat
SKU: 34022 Categories: , ,
Overview
Product Name Anti-BRCAA1 Antibody (34022)
Description Anti-BRCAA1 Rabbit Polyclonal Antibody
Target BRCAA1
Species Reactivity Human, Mouse, Rat
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to amino acids 1046- 1055 (SSKKQKRSHK) of human BRCAA1 conjugated to KLH.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat BRCAA1.
Target Name AT-rich interactive domain-containing protein 4B
Target ID BRCAA1
Uniprot ID Q4LE39
Alternative Names ARID domain-containing protein 4B, 180 kDa Sin3-associated polypeptide, Sin3-associated polypeptide p180, Breast cancer-associated antigen BRCAA1, Histone deacetylase complex subunit SAP180, Retinoblastoma-binding protein 1-like 1
Gene Name ARID4B
Sequence Location Nucleus, Cytoplasm
Biological Function Acts as a transcriptional repressor (PubMed:12724404). May function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes (PubMed:12724404). Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4A. Involved in spermatogenesis, together with ARID4A, where it functions as a transcriptional coactivator for AR (androgen receptor) and enhances expression of genes required for sperm maturation. Regulates expression of the tight junction protein CLDN3 in the testis, which is important for integrity of the blood-testis barrier. Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis. May function as a leukemia suppressor (By similarity). {UniProtKB:A2CG63, PubMed:12724404}.
Research Areas Cancer research
Background The novel peptide sequence SSKKQKRSHK within BRCAA1 protein was mapped to the cytoplasm of MCF-7 cells. Immunohistochemical analysis demonstrated that this sequence was expressed in 65% of breast tumors. Its expression may be useful in diagnosis and prognosis of breast cancer.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting : use at 1:500-1:1,000 dilution. A band of approximately 136 kDa is detected.

Positive control: MCF-7 human breast cancer cell lysate.
References & Data Sheet
References Cui, D et al. (2004) Cancer Epidemiol Biomarkers Pres 13: 1136-45.