Anti-BRCA1 Antibody

$461.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF,IHC-P,IP,ELISA,ChIP,IHC Human
SKU: 3301 Categories: , ,
Overview
Product Name Anti-BRCA1 Antibody (3301)
Description Anti-BRCA1 Mouse Monoclonal Antibody
Target BRCA1
Species Reactivity Human
Applications WB,ICC/IF,IHC-P,IP,ELISA,ChIP,IHC
Host Mouse
Clonality Monoclonal
Clone ID 1D12
Isotype IgG1
Immunogen GST fusion protein expressed in E. coli corresponding to aa 341-748 of full-length BRCA1.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes full-length BRCA1, a 220 kD nuclear phosphoprotein. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.
Target Name Breast cancer type 1 susceptibility protein
Target ID BRCA1
Uniprot ID P38398
Alternative Names EC 2.3.2.27, RING finger protein 53, RING-type E3 ubiquitin transferase BRCA1
Gene Name BRCA1
Gene ID 672
Accession Number NP_009225
Sequence Location Nucleus, Chromosome, Cytoplasm
Biological Function E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage (PubMed:12890688, PubMed:14976165, PubMed:16818604, PubMed:17525340, PubMed:12887909, PubMed:10500182, PubMed:19261748). It is unclear whether it also mediates the formation of other types of polyubiquitin chains (PubMed:12890688). The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability (PubMed:12890688, PubMed:14976165, PubMed:20351172). Regulates centrosomal microtubule nucleation (PubMed:18056443). Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle (PubMed:10724175, PubMed:12183412, PubMed:11836499, PubMed:19261748). Required for FANCD2 targeting to sites of DNA damage (PubMed:12887909). Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation (PubMed:16326698). Contributes to homologous rPubMed:10500182, PubMed:10724175, PubMed:11836499, PubMed:12183412, PubMed:12887909, PubMed:12890688, PubMed:14976165, PubMed:16326698, PubMed:16818604, PubMed:17525340, PubMed:18056443, PubMed:19261748, PubMed:19369211, PubMed:20160719, PubMed:20351172}.
Research Areas Cancer Research
Application Images
Description BRCA1 antibody [17F8] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade (3301) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Description BRCA1 antibody 17F8 (3301) was used for IP-WB assay.
6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract.
Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution.
Description BRCA1 antibody 17F8 (3301) were used for ChIP assay.
The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay.
Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.
Additional Information
Handling
Storage Store at 2 - 8ºC. Do not Freeze.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-10 ug/mL.

Immunoprecipitation: use at 1-10 ug/mL.

Positive controls: Any normal human tissue, especially those containing rapidly proliferating cells, such as lymphoid germinal centers.
References & Data Sheet
References Chen, Y et al. (1995) Science 270: 789- 791; Chen, C-F et al. (1995) J Biol Chem 271: 32863-32868; Chen, Y et al. (1996) Cancer Res 56: 3168-3172.