APP (Abeta-NT)

Anti-APP (Aβ-NT) Antibody

ORDERING INFORMATION

Catalog No.: 2133
Size: 100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chroma-tography.

BACKGROUND

Accumulation of the amyloid-β peptide (Aβ) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The β-amyloid protein precursor (APP) is cleaved by β-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for γ-secretase to generate the 4 kD amyloid-β peptide which is deposited in the brain.

SPECIFICITY

This antibody recognizes human, mouse, and rat APP and Aβ peptide.

SPECIFICATION SUMMARY

Antigen: Peptide corresponding to aa 653- 662 of human amyloid protein precursor (APP) or aa 1-10 of the 4kD Aβ peptide generated by β- and γ-secretases. The sequences are identical to those of rabbit, pig, cow, guinea pig, and chicken.
Host Species: Rabbit
Stabilizers: None
Preservatives: 0.02% sodium azide.

APPLICATIONS

Immunoblotting: use at 1:500-1:1,000 dilution.
Positive control: Mouse brain lysate.

DILUTION INSTRUCTIONS

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE AND STABILITY

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.