Anti-Apaf1 (CT) Antibody
Catalog No.: 2015
Size: 100ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease- activating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.
Antigen: Peptide corresponding to aa 1158- 1177 of human Apaf1. This sequence differs from that of mouse Apaf1 by one amino acid.
Host Species: Rabbit
Preservatives: 0.02% sodium azide
This antibody recognizes human, mouse, and rat Apaf1 (130kDa).
Immunoblotting: use at 1-10ug/ml
Immunohistochemistry: use at 10-20ug/ml
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Positive control: Human heart tissue lysate.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.